Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds

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Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds. / Liu, Shu Jun; Xu, Heng Heng; Wang, Wei Qing; Li, Ni; Wang, Wei Ping; Lu, Zhuang; Møller, Ian Max; Song, Song Quan.

In: Journal of Plant Physiology, Vol. 196-197, 01.06.2016, p. 79-92.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Liu, SJ, Xu, HH, Wang, WQ, Li, N, Wang, WP, Lu, Z, Møller, IM & Song, SQ 2016, 'Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds', Journal of Plant Physiology, vol. 196-197, pp. 79-92. https://doi.org/10.1016/j.jplph.2016.02.021

APA

Liu, S. J., Xu, H. H., Wang, W. Q., Li, N., Wang, W. P., Lu, Z., ... Song, S. Q. (2016). Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds. Journal of Plant Physiology, 196-197, 79-92. https://doi.org/10.1016/j.jplph.2016.02.021

CBE

MLA

Vancouver

Liu SJ, Xu HH, Wang WQ, Li N, Wang WP, Lu Z et al. Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds. Journal of Plant Physiology. 2016 Jun 1;196-197:79-92. https://doi.org/10.1016/j.jplph.2016.02.021

Author

Liu, Shu Jun ; Xu, Heng Heng ; Wang, Wei Qing ; Li, Ni ; Wang, Wei Ping ; Lu, Zhuang ; Møller, Ian Max ; Song, Song Quan. / Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds. In: Journal of Plant Physiology. 2016 ; Vol. 196-197. pp. 79-92.

Bibtex

@article{f15c14c128eb47599286e265a02cef86,
title = "Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds",
abstract = "Seed germination is a critical phase in the plant life cycle, but the mechanism of seed germination is still poorly understood. In the present study, rice (Oryza sativa L. cv. Peiai 64S) seeds were sampled individually when they reached different germination stages, quiescent, germinated sensu stricto, germinated completely and seedling, and were used to study the changes in the embryo proteome. A total of 88 protein spots showed a significant change in abundance during germination in water, and the results showed an activation of metabolic processes. Cell division, cell wall synthesis, and secondary metabolism were activated at late seed germination and during preparation for subsequent seedling establishment. Cycloheximide (CHX) at 70 μM inhibited seedling establishment without an apparent negative effect on seed germination, while CHX at 500 μM completely blocked seed germination. We used this observation to identify the potentially important proteins involved in seed germination (coleoptile protrusion) and seedling establishment (coleoptile and radicle protrusion). Twenty-six protein spots, mainly associated with sugar/polysaccharide metabolism and energy production, showed a significant difference in abundance during seed germination. Forty-nine protein spots, mainly involved in cell wall biosynthesis, proteolysis as well as cell defense and rescue, were required for seedling establishment. The results help improve our understanding of the key events (proteins) involved in germination and seedling development.",
keywords = "Embryo, Oryza sativa, Proteome analysis, Seed germination, Seedling establishment",
author = "Liu, {Shu Jun} and Xu, {Heng Heng} and Wang, {Wei Qing} and Ni Li and Wang, {Wei Ping} and Zhuang Lu and M{\o}ller, {Ian Max} and Song, {Song Quan}",
year = "2016",
month = "6",
day = "1",
doi = "10.1016/j.jplph.2016.02.021",
language = "English",
volume = "196-197",
pages = "79--92",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Elsevier GmbH - Urban und Fischer",

}

RIS

TY - JOUR

T1 - Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds

AU - Liu, Shu Jun

AU - Xu, Heng Heng

AU - Wang, Wei Qing

AU - Li, Ni

AU - Wang, Wei Ping

AU - Lu, Zhuang

AU - Møller, Ian Max

AU - Song, Song Quan

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Seed germination is a critical phase in the plant life cycle, but the mechanism of seed germination is still poorly understood. In the present study, rice (Oryza sativa L. cv. Peiai 64S) seeds were sampled individually when they reached different germination stages, quiescent, germinated sensu stricto, germinated completely and seedling, and were used to study the changes in the embryo proteome. A total of 88 protein spots showed a significant change in abundance during germination in water, and the results showed an activation of metabolic processes. Cell division, cell wall synthesis, and secondary metabolism were activated at late seed germination and during preparation for subsequent seedling establishment. Cycloheximide (CHX) at 70 μM inhibited seedling establishment without an apparent negative effect on seed germination, while CHX at 500 μM completely blocked seed germination. We used this observation to identify the potentially important proteins involved in seed germination (coleoptile protrusion) and seedling establishment (coleoptile and radicle protrusion). Twenty-six protein spots, mainly associated with sugar/polysaccharide metabolism and energy production, showed a significant difference in abundance during seed germination. Forty-nine protein spots, mainly involved in cell wall biosynthesis, proteolysis as well as cell defense and rescue, were required for seedling establishment. The results help improve our understanding of the key events (proteins) involved in germination and seedling development.

AB - Seed germination is a critical phase in the plant life cycle, but the mechanism of seed germination is still poorly understood. In the present study, rice (Oryza sativa L. cv. Peiai 64S) seeds were sampled individually when they reached different germination stages, quiescent, germinated sensu stricto, germinated completely and seedling, and were used to study the changes in the embryo proteome. A total of 88 protein spots showed a significant change in abundance during germination in water, and the results showed an activation of metabolic processes. Cell division, cell wall synthesis, and secondary metabolism were activated at late seed germination and during preparation for subsequent seedling establishment. Cycloheximide (CHX) at 70 μM inhibited seedling establishment without an apparent negative effect on seed germination, while CHX at 500 μM completely blocked seed germination. We used this observation to identify the potentially important proteins involved in seed germination (coleoptile protrusion) and seedling establishment (coleoptile and radicle protrusion). Twenty-six protein spots, mainly associated with sugar/polysaccharide metabolism and energy production, showed a significant difference in abundance during seed germination. Forty-nine protein spots, mainly involved in cell wall biosynthesis, proteolysis as well as cell defense and rescue, were required for seedling establishment. The results help improve our understanding of the key events (proteins) involved in germination and seedling development.

KW - Embryo

KW - Oryza sativa

KW - Proteome analysis

KW - Seed germination

KW - Seedling establishment

UR - http://www.scopus.com/inward/record.url?scp=84962822269&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2016.02.021

DO - 10.1016/j.jplph.2016.02.021

M3 - Journal article

C2 - 27085178

AN - SCOPUS:84962822269

VL - 196-197

SP - 79

EP - 92

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

ER -