Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds

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  • Shu Jun Liu, Institute of Botany, Chinese Academy of Sciences
  • ,
  • Heng Heng Xu, Institute of Botany, Chinese Academy of Sciences
  • ,
  • Wei Qing Wang, Institute of Botany, Chinese Academy of Sciences
  • ,
  • Ni Li, Hunan Hybrid Rice Research Center/State Key Laboratory of Hybrid Rice
  • ,
  • Wei Ping Wang, Hunan Hybrid Rice Research Center/State Key Laboratory of Hybrid Rice
  • ,
  • Zhuang Lu, Institute of Botany, Chinese Academy of Sciences
  • ,
  • Ian Max Møller
  • Song Quan Song, Institute of Botany, Chinese Academy of Sciences

Seed germination is a critical phase in the plant life cycle, but the mechanism of seed germination is still poorly understood. In the present study, rice (Oryza sativa L. cv. Peiai 64S) seeds were sampled individually when they reached different germination stages, quiescent, germinated sensu stricto, germinated completely and seedling, and were used to study the changes in the embryo proteome. A total of 88 protein spots showed a significant change in abundance during germination in water, and the results showed an activation of metabolic processes. Cell division, cell wall synthesis, and secondary metabolism were activated at late seed germination and during preparation for subsequent seedling establishment. Cycloheximide (CHX) at 70 μM inhibited seedling establishment without an apparent negative effect on seed germination, while CHX at 500 μM completely blocked seed germination. We used this observation to identify the potentially important proteins involved in seed germination (coleoptile protrusion) and seedling establishment (coleoptile and radicle protrusion). Twenty-six protein spots, mainly associated with sugar/polysaccharide metabolism and energy production, showed a significant difference in abundance during seed germination. Forty-nine protein spots, mainly involved in cell wall biosynthesis, proteolysis as well as cell defense and rescue, were required for seedling establishment. The results help improve our understanding of the key events (proteins) involved in germination and seedling development.

Original languageEnglish
JournalJournal of Plant Physiology
Volume196-197
Pages (from-to)79-92
Number of pages14
ISSN0176-1617
DOIs
Publication statusPublished - 1 Jun 2016

    Research areas

  • Embryo, Oryza sativa, Proteome analysis, Seed germination, Seedling establishment

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