Identification and developmental analysis of endothelin receptor type-A expressing cells in the mouse kidney

TY - JOUR

T1 - Identification and developmental analysis of endothelin receptor type-A expressing cells in the mouse kidney

AU - Kitazawa, Taro

AU - Sato, Takahiro

AU - Nishiyama, Koichi

AU - Asai, Rieko

AU - Arima, Yuichiro

AU - Uchijima, Yasunobu

AU - Kurihara, Yukiko

AU - Kurihara, Hiroki

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011/10

Y1 - 2011/10

N2 - The endothelin (Edn) system plays pleiotropic roles in renal function and various disease processes through two distinct G protein-coupled receptors, Edn receptors type-A (Ednra) and type-B (Ednrb). However, difficulties in the accurate identification of receptor-expressing cells in situ have made it difficult to dissect their diverse action in renal (patho)physiology. We have recently established mouse lines in which lacZ and EGFP are 'knocked-in' to the Ednra locus to faithfully mark Ednra-expressing cells. Here we analyzed these mice for their expression in the kidney to characterize Ednra-expressing cells. Ednra expression was first observed in undifferentiated mesenchymal cells around the ureteric bud at E12.5. Thereafter, Ednra expression was widely observed in vascular smooth muscle cells, JG cells and mesenchymal cells in the interstitium. After growth, the expression became confined to vascular smooth muscle cells, pericytes and renin-producing JG cells. By contrast, most cells in the nephron and vascular endothelial cells did not express Ednra. These results indicate that Ednra expression may be linked with non-epithelial fate determination and differentiation of metanephric mesenchyme. Ednra-lacZ/EGFP knock-in mice may serve as a useful tool in studies on renal function and pathophysiology of various renal diseases.

AB - The endothelin (Edn) system plays pleiotropic roles in renal function and various disease processes through two distinct G protein-coupled receptors, Edn receptors type-A (Ednra) and type-B (Ednrb). However, difficulties in the accurate identification of receptor-expressing cells in situ have made it difficult to dissect their diverse action in renal (patho)physiology. We have recently established mouse lines in which lacZ and EGFP are 'knocked-in' to the Ednra locus to faithfully mark Ednra-expressing cells. Here we analyzed these mice for their expression in the kidney to characterize Ednra-expressing cells. Ednra expression was first observed in undifferentiated mesenchymal cells around the ureteric bud at E12.5. Thereafter, Ednra expression was widely observed in vascular smooth muscle cells, JG cells and mesenchymal cells in the interstitium. After growth, the expression became confined to vascular smooth muscle cells, pericytes and renin-producing JG cells. By contrast, most cells in the nephron and vascular endothelial cells did not express Ednra. These results indicate that Ednra expression may be linked with non-epithelial fate determination and differentiation of metanephric mesenchyme. Ednra-lacZ/EGFP knock-in mice may serve as a useful tool in studies on renal function and pathophysiology of various renal diseases.

KW - Animals

KW - Cell Differentiation

KW - Gene Expression Regulation

KW - Gene Knock-In Techniques

KW - Green Fluorescent Proteins/genetics

KW - Kidney/cytology

KW - Mesoderm/cytology

KW - Mice

KW - Mice, Transgenic

KW - Muscle, Smooth, Vascular/cytology

KW - Nephrons/cytology

KW - Pericytes/cytology

KW - Receptor, Endothelin A/genetics

U2 - 10.1016/j.gep.2011.04.001

DO - 10.1016/j.gep.2011.04.001

M3 - Journal article

C2 - 21565284

SN - 1567-133X

VL - 11

SP - 371

EP - 377

JO - Gene Expression Patterns

JF - Gene Expression Patterns

IS - 7

ER -