Hyperpolarized 13c magnetic resonance spectroscopy and hypoxic ischemic encephalopathy – proof of concept in a piglet model

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Background and aims:
Biomarkers of cerebral damage are key for prognostication in newborn with hypoxic ischemic encephalopathy (HIE). Hyperpolarized 13C magnetic resonance spectroscopy (MRS) is a novel method to quantify metabolism in vivo with ultra-high resolution. In this proof-of-concept study, we aimed to investigate whether hyperpolarized 13C MRS can be applied in a piglet model of HIE and used to detect early changes in metabolite profile after a standardized hypoxic ischemic (HI) insult.

Six piglets were anesthetised and subjected to a standardised HI insult. Imaging was performed at baseline and two hours after the HI insult on a 3 T MR scanner. For 13C studies, 127 mg [1-13C]pyruvic acid was hyperpolarized in the SpinLab polarizer. Following intra venous injection (6 ml), rapid interleaved spectroscopic images were acquired, using a spectral-spatial spiral imaging sequence. Metabolism was quantified as area under the curve and determined for pyruvate, alanine, lactate, and bicarbonate.

Hyperpolarized 13C MRS was successfully performed in all animals. HI resulted in a reduction in bicarbonate metabolism (mean (SD); 116.5 (25.7) vs. 37.6 (5.9), p = 0.02). There was no change in lactate (576.6 (103.9) vs. 547.2 (100.3), p = 0.50), alanine (74.4 (12.0) vs. 68.6 (31.3), p = 0.84), or pyruvate (391.6 (69.6) vs. 263.9 (80.6), p = 0.06) at two hours.

For the first time ever, we show that hyperpolarized 13C MRS can be used in the piglet and applied to evaluate early changes in cerebral metabolism after a HI insult.
Original languageEnglish
Publication year2020
Publication statusPublished - 2020
Event8th Congress of the European Academy of Paediatric Societies -
Duration: 16 Oct 202019 Oct 2020


Conference8th Congress of the European Academy of Paediatric Societies

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