Abstract
Early biomarkers of cerebral damage are essential for accurate prognosis, timely intervention, and evaluation of new treatment modalities in newborn infants with hypoxia and ischemia at birth. Hyperpolarized 13 C magnetic resonance imaging (MRI) is a novel method with which to quantify metabolism in vivo with unprecedented sensitivity. We aimed to investigate the applicability of hyperpolarized 13 C MRI in a newborn piglet model and whether this method may identify early changes in cerebral metabolism after a standardized hypoxic-ischemic (HI) insult. Six piglets were anesthetized and subjected to a standardized HI insult. Imaging was performed prior to and 2 h after the insult on a 3-T MR scanner. For 13 C studies, [1- 13 C]pyruvate was hyperpolarized in a commercial polarizer. Following intravenous injection, images were acquired using metabolic-specific imaging. HI resulted in a metabolic shift with a decrease in pyruvate to bicarbonate metabolism and an increase in pyruvate to lactate metabolism (lactate/bicarbonate ratio, mean [SD]; 2.28 [0.36] vs. 3.96 [0.91]). This is the first study to show that hyperpolarized 13 C MRI can be used in newborn piglets and applied to evaluate early changes in cerebral metabolism after an HI insult.
Original language | English |
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Article number | e5110 |
Journal | NMR in Biomedicine |
Volume | 37 |
Issue | 5 |
Pages (from-to) | e5110 |
ISSN | 1099-1492 |
DOIs | |
Publication status | Published - May 2024 |
Keywords
- animal model
- hyperpolarized MRI
- hypoxic ischemic encephalopathy
- neonatology
- Humans
- Bicarbonates
- Infant
- Magnetic Resonance Imaging/methods
- Hypoxia-Ischemia, Brain/diagnostic imaging
- Animals
- Swine
- Hypoxia
- Lactic Acid/metabolism
- Models, Animal
- Pyruvic Acid/metabolism
- Infant, Newborn