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Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens

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Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens. / Borup-Christensen, P; Erb, Karin; Ditzel, H; Nielsen, B; Larsen, J K; Svehag, S E; Jensenius, Jens Christian.

In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Vol. 98, No. 8, 1990, p. 674-84.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Borup-Christensen, P, Erb, K, Ditzel, H, Nielsen, B, Larsen, JK, Svehag, SE & Jensenius, JC 1990, 'Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens', A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, vol. 98, no. 8, pp. 674-84.

APA

Borup-Christensen, P., Erb, K., Ditzel, H., Nielsen, B., Larsen, J. K., Svehag, S. E., & Jensenius, J. C. (1990). Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, 98(8), 674-84.

CBE

Borup-Christensen P, Erb K, Ditzel H, Nielsen B, Larsen JK, Svehag SE, Jensenius JC. 1990. Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 98(8):674-84.

MLA

Borup-Christensen, P et al. "Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens". A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 1990, 98(8). 674-84.

Vancouver

Borup-Christensen P, Erb K, Ditzel H, Nielsen B, Larsen JK, Svehag SE et al. Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 1990;98(8):674-84.

Author

Borup-Christensen, P ; Erb, Karin ; Ditzel, H ; Nielsen, B ; Larsen, J K ; Svehag, S E ; Jensenius, Jens Christian. / Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens. In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 1990 ; Vol. 98, No. 8. pp. 674-84.

Bibtex

@article{684d1c8da4d048a18caefc5006cd28e0,
title = "Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens",
abstract = "Lymphocytes from lymph nodes draining the tumor region in patients with colorectal cancer were fused with two different human B-lymphoblastoid cell lines, LICR-LON-HMy-2 (HMy-2) and WI-L2-729-HF2 (729-HF2), to generate hybridomas synthesizing antibodies reacting with tumor-associated antigens. In this way 220 hybridomas were obtained which produce antibody reacting with colon cancer cells. All established clones produced IgM. Four human monoclonal antibodies have been further analyzed. The cell lines producing these antibodies are all hybrids based on DNA analysis. Three of the antibodies (G4146, B9165 and D4213) showed binding to differentiation antigens by immunocytochemical analysis on different cancer cell lines and normal human leucocytes and by immunohistochemical analysis on sections of frozen malignant and normal tissues, while the fourth (F11348) showed a reaction with all cells and tissues tested. Western blots of tumor extracts showed binding of G4146 to two components from colon cancer cells with Mr of 59 K and 61 K, while B9165 bound to a 43 K component and F11348 to several components with Mr from 30 to 200K. D4213 showed no binding in this analysis. The results obtained demonstrate the successful application of hybridoma technology to produce human monoclonals with reactivity to differentiation antigens.",
keywords = "Antibodies, Monoclonal, Antigens, Neoplasm, Blotting, Western, Cell Fusion, Colorectal Neoplasms, Epithelium, Flow Cytometry, Humans, Hybridomas, Immunoglobulin M, Immunohistochemistry, Molecular Weight",
author = "P Borup-Christensen and Karin Erb and H Ditzel and B Nielsen and Larsen, {J K} and Svehag, {S E} and Jensenius, {Jens Christian}",
year = "1990",
language = "English",
volume = "98",
pages = "674--84",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "8",

}

RIS

TY - JOUR

T1 - Human-human hybridoma producing monoclonal antibodies against colorectal cancer-associated antigens

AU - Borup-Christensen, P

AU - Erb, Karin

AU - Ditzel, H

AU - Nielsen, B

AU - Larsen, J K

AU - Svehag, S E

AU - Jensenius, Jens Christian

PY - 1990

Y1 - 1990

N2 - Lymphocytes from lymph nodes draining the tumor region in patients with colorectal cancer were fused with two different human B-lymphoblastoid cell lines, LICR-LON-HMy-2 (HMy-2) and WI-L2-729-HF2 (729-HF2), to generate hybridomas synthesizing antibodies reacting with tumor-associated antigens. In this way 220 hybridomas were obtained which produce antibody reacting with colon cancer cells. All established clones produced IgM. Four human monoclonal antibodies have been further analyzed. The cell lines producing these antibodies are all hybrids based on DNA analysis. Three of the antibodies (G4146, B9165 and D4213) showed binding to differentiation antigens by immunocytochemical analysis on different cancer cell lines and normal human leucocytes and by immunohistochemical analysis on sections of frozen malignant and normal tissues, while the fourth (F11348) showed a reaction with all cells and tissues tested. Western blots of tumor extracts showed binding of G4146 to two components from colon cancer cells with Mr of 59 K and 61 K, while B9165 bound to a 43 K component and F11348 to several components with Mr from 30 to 200K. D4213 showed no binding in this analysis. The results obtained demonstrate the successful application of hybridoma technology to produce human monoclonals with reactivity to differentiation antigens.

AB - Lymphocytes from lymph nodes draining the tumor region in patients with colorectal cancer were fused with two different human B-lymphoblastoid cell lines, LICR-LON-HMy-2 (HMy-2) and WI-L2-729-HF2 (729-HF2), to generate hybridomas synthesizing antibodies reacting with tumor-associated antigens. In this way 220 hybridomas were obtained which produce antibody reacting with colon cancer cells. All established clones produced IgM. Four human monoclonal antibodies have been further analyzed. The cell lines producing these antibodies are all hybrids based on DNA analysis. Three of the antibodies (G4146, B9165 and D4213) showed binding to differentiation antigens by immunocytochemical analysis on different cancer cell lines and normal human leucocytes and by immunohistochemical analysis on sections of frozen malignant and normal tissues, while the fourth (F11348) showed a reaction with all cells and tissues tested. Western blots of tumor extracts showed binding of G4146 to two components from colon cancer cells with Mr of 59 K and 61 K, while B9165 bound to a 43 K component and F11348 to several components with Mr from 30 to 200K. D4213 showed no binding in this analysis. The results obtained demonstrate the successful application of hybridoma technology to produce human monoclonals with reactivity to differentiation antigens.

KW - Antibodies, Monoclonal

KW - Antigens, Neoplasm

KW - Blotting, Western

KW - Cell Fusion

KW - Colorectal Neoplasms

KW - Epithelium

KW - Flow Cytometry

KW - Humans

KW - Hybridomas

KW - Immunoglobulin M

KW - Immunohistochemistry

KW - Molecular Weight

M3 - Journal article

C2 - 2206514

VL - 98

SP - 674

EP - 684

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 8

ER -