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Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination

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Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination. / Jensen, S; Redwood, C S; Jenkins, J R; Andersen, A H; Hickson, I D.

In: Molecular Genetics and Genomics, Vol. 252, No. 1-2, 27.08.1996, p. 79-86.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Jensen, S, Redwood, CS, Jenkins, JR, Andersen, AH & Hickson, ID 1996, 'Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination', Molecular Genetics and Genomics, vol. 252, no. 1-2, pp. 79-86.

APA

Jensen, S., Redwood, C. S., Jenkins, J. R., Andersen, A. H., & Hickson, I. D. (1996). Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination. Molecular Genetics and Genomics, 252(1-2), 79-86.

CBE

Jensen S, Redwood CS, Jenkins JR, Andersen AH, Hickson ID. 1996. Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination. Molecular Genetics and Genomics. 252(1-2):79-86.

MLA

Vancouver

Jensen S, Redwood CS, Jenkins JR, Andersen AH, Hickson ID. Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination. Molecular Genetics and Genomics. 1996 Aug 27;252(1-2):79-86.

Author

Jensen, S ; Redwood, C S ; Jenkins, J R ; Andersen, A H ; Hickson, I D. / Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination. In: Molecular Genetics and Genomics. 1996 ; Vol. 252, No. 1-2. pp. 79-86.

Bibtex

@article{70eb0a0f6e2841e090ad07876f21f529,
title = "Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination",
abstract = "The ability of the human DNA topoisomerase II alpha and II beta isozymes to complement functional defects conferred by conditional top2 mutations in Saccharomyces cerevisiae has been investigated. At the restrictive temperature, top2 strains show multiple abnormalities, including an inability to complete mitotic and meiotic division owing to a defect in chromosome segregation, and hyper-recombination within the repetitive rDNA gene cluster. We show that the human topoisomerases II alpha and II beta can each support both vegetative growth and the production of viable spores in a top2-4 mutant at the restrictive temperature. Similarly, both human isozymes can rescue a strain carrying a top2 gene disruption, and suppress hyper-recombination within the rDNA gene cluster. We conclude that the human topoisomerase II alpha and II beta isozymes are functionally interchangeable with yeast topoisomerase II and suggest that any isozyme-specific roles in human cells are likely to be dependent upon factors other than inherent differences in catalytic ability between the alpha and beta isozymes.",
keywords = "Antigens, Neoplasm, DNA Topoisomerases, Type II, DNA-Binding Proteins, Gene Expression, Genetic Complementation Test, Humans, Isoenzymes, Meiosis, Mutation, Recombination, Genetic, Saccharomyces cerevisiae, Spores, Fungal, Temperature, Transformation, Genetic, Journal Article, Research Support, Non-U.S. Gov't",
author = "S Jensen and Redwood, {C S} and Jenkins, {J R} and Andersen, {A H} and Hickson, {I D}",
year = "1996",
month = aug,
day = "27",
language = "English",
volume = "252",
pages = "79--86",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer",
number = "1-2",

}

RIS

TY - JOUR

T1 - Human DNA topoisomerases II alpha and II beta can functionally substitute for yeast TOP2 in chromosome segregation and recombination

AU - Jensen, S

AU - Redwood, C S

AU - Jenkins, J R

AU - Andersen, A H

AU - Hickson, I D

PY - 1996/8/27

Y1 - 1996/8/27

N2 - The ability of the human DNA topoisomerase II alpha and II beta isozymes to complement functional defects conferred by conditional top2 mutations in Saccharomyces cerevisiae has been investigated. At the restrictive temperature, top2 strains show multiple abnormalities, including an inability to complete mitotic and meiotic division owing to a defect in chromosome segregation, and hyper-recombination within the repetitive rDNA gene cluster. We show that the human topoisomerases II alpha and II beta can each support both vegetative growth and the production of viable spores in a top2-4 mutant at the restrictive temperature. Similarly, both human isozymes can rescue a strain carrying a top2 gene disruption, and suppress hyper-recombination within the rDNA gene cluster. We conclude that the human topoisomerase II alpha and II beta isozymes are functionally interchangeable with yeast topoisomerase II and suggest that any isozyme-specific roles in human cells are likely to be dependent upon factors other than inherent differences in catalytic ability between the alpha and beta isozymes.

AB - The ability of the human DNA topoisomerase II alpha and II beta isozymes to complement functional defects conferred by conditional top2 mutations in Saccharomyces cerevisiae has been investigated. At the restrictive temperature, top2 strains show multiple abnormalities, including an inability to complete mitotic and meiotic division owing to a defect in chromosome segregation, and hyper-recombination within the repetitive rDNA gene cluster. We show that the human topoisomerases II alpha and II beta can each support both vegetative growth and the production of viable spores in a top2-4 mutant at the restrictive temperature. Similarly, both human isozymes can rescue a strain carrying a top2 gene disruption, and suppress hyper-recombination within the rDNA gene cluster. We conclude that the human topoisomerase II alpha and II beta isozymes are functionally interchangeable with yeast topoisomerase II and suggest that any isozyme-specific roles in human cells are likely to be dependent upon factors other than inherent differences in catalytic ability between the alpha and beta isozymes.

KW - Antigens, Neoplasm

KW - DNA Topoisomerases, Type II

KW - DNA-Binding Proteins

KW - Gene Expression

KW - Genetic Complementation Test

KW - Humans

KW - Isoenzymes

KW - Meiosis

KW - Mutation

KW - Recombination, Genetic

KW - Saccharomyces cerevisiae

KW - Spores, Fungal

KW - Temperature

KW - Transformation, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 8804406

VL - 252

SP - 79

EP - 86

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 1-2

ER -