TY - JOUR
T1 - Human dental pulp cells exhibit bone cell-like responsiveness to fluid shear stress
AU - Kraft, David Christian Evar
AU - Bindslev, Dorthe Arenholt
AU - Melsen, Birte
AU - Klein-Nulend, Jenneke
PY - 2011
Y1 - 2011
N2 - Abstract Background aims. For engineering bone tissue to restore, for example, maxillofacial defects, mechanosensitive cells are needed that are able to conduct bone cell-specific functions, such as bone remodelling. Mechanical loading affects local bone mass and architecture in vivo by initiating a cellular response via loading-induced flow of interstitial fluid. After surgical removal of ectopically impacted third molars, human dental pulp tissue is an easily accessible and interesting source of cells for mineralized tissue engineering. The aim of this study was to determine whether human dental pulp-derived cells (DPC) are responsive to mechanical loading by pulsating fluid flow (PFF) upon stimulation of mineralization in vitro. Methods. Human DPC were incubated with or without mineralization medium containing differentiation factors for 3 weeks. Cells were subjected to 1-h PFF (0.7 +/- 0.3Pa, 5Hz) and the response was quantified by measuring nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production, and gene expression of cyclooxygenase (COX)-1 and COX-2. Results. We found that DPC are intrinsically mechanosensitive and, like osteogenic cells, respond to PFF-induced fluid shear stress. PFF stimulated NO and PGE(2) production, and up-regulated COX-2 but not COX-1 gene expression. In DPC cultured under mineralizing conditions, the PFF-induced NO, but not PGE(2), production was significantly enhanced. Conclusions. These data suggest that human DPC, like osteogenic cells, acquire responsiveness to pulsating fluid shear stress in mineralizing conditions. Thus DPC might be able to perform bone-like functions during mineralized tissue remodeling in vivo, and therefore provide a promising new tool for mineralized tissue engineering to restore, for example, maxillofacial defects.
AB - Abstract Background aims. For engineering bone tissue to restore, for example, maxillofacial defects, mechanosensitive cells are needed that are able to conduct bone cell-specific functions, such as bone remodelling. Mechanical loading affects local bone mass and architecture in vivo by initiating a cellular response via loading-induced flow of interstitial fluid. After surgical removal of ectopically impacted third molars, human dental pulp tissue is an easily accessible and interesting source of cells for mineralized tissue engineering. The aim of this study was to determine whether human dental pulp-derived cells (DPC) are responsive to mechanical loading by pulsating fluid flow (PFF) upon stimulation of mineralization in vitro. Methods. Human DPC were incubated with or without mineralization medium containing differentiation factors for 3 weeks. Cells were subjected to 1-h PFF (0.7 +/- 0.3Pa, 5Hz) and the response was quantified by measuring nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production, and gene expression of cyclooxygenase (COX)-1 and COX-2. Results. We found that DPC are intrinsically mechanosensitive and, like osteogenic cells, respond to PFF-induced fluid shear stress. PFF stimulated NO and PGE(2) production, and up-regulated COX-2 but not COX-1 gene expression. In DPC cultured under mineralizing conditions, the PFF-induced NO, but not PGE(2), production was significantly enhanced. Conclusions. These data suggest that human DPC, like osteogenic cells, acquire responsiveness to pulsating fluid shear stress in mineralizing conditions. Thus DPC might be able to perform bone-like functions during mineralized tissue remodeling in vivo, and therefore provide a promising new tool for mineralized tissue engineering to restore, for example, maxillofacial defects.
U2 - 10.3109/14653249.2010.487897
DO - 10.3109/14653249.2010.487897
M3 - Journal article
C2 - 20491534
SN - 1465-3249
VL - 13
SP - 214
EP - 226
JO - Cytotherapy
JF - Cytotherapy
ER -