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Human autoantibodies against Clq: lack of cross reactivity with the collectins mannan-binding protein, lung surfactant protein A and bovine conglutinin

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  • Department of Medical Microbiology and Immunology
The collectins, a group of humoral C-type lectins, have globular and collagen-like regions and share structural features with the complement protein C1q. The question was asked if autoantibodies to the collagen-like region of C1q (anti-C1qCLR) might cross-react with collectins, such as mannan-binding protein (MBP), lung surfactant protein A (SP-A) and bovine conglutinin (BK). Anti-C1qCLR antibodies of the systemic lupus erythematosus (SLE) type and anti-C1qCLR antibodies of the hypocomplementemic urticarial vasculitis syndrome (HUVS) type were investigated. Cross-absorption and elution experiments combined with antibody detection by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis gave no evidence of cross-reactive anti-C1qCLR antibodies. However, one serum with HUVS type anti-C1qCLR antibodies contained anti-MBP antibodies that were cross-reactive with SP-A. Judging from results of ELISA inhibition experiments and immunoblot analysis, four SLE sera contained antibodies to native BK, while two sera with HUVS type anti-C1qCLR antibodies contained antibodies to epitopes of denatured BK. This might imply that autoimmunity to collagen-like structures is not restricted to C1qCLR in HUVS and HUVS/SLE overlap syndromes.
Original languageEnglish
JournalScandinavian Journal of Immunology
Pages (from-to)314-20
Number of pages7
Publication statusPublished - 1 Mar 1996

    Research areas

  • Animals, Autoantibodies, Binding Sites, Antibody, Binding, Competitive, Carrier Proteins, Cattle, Collectins, Complement C1q, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Glycoproteins, Humans, Immunoblotting, Lectins, Mannans, Proteolipids, Pulmonary Surfactant-Associated Protein A, Pulmonary Surfactant-Associated Proteins, Pulmonary Surfactants, Serum Globulins

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