HTLV infected individuals have increased B-cell activation and proinflammatory regulatory T-cells

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HTLV infected individuals have increased B-cell activation and proinflammatory regulatory T-cells. / Kjerulff, Bertram; Petersen, Mikkel Steen; Rodrigues, Candida Medina et al.

In: Immunobiology, Vol. 225, No. 2, 151878, 2020.

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@article{ff589bea852041b9bc2bb3a9c9d45041,
title = "HTLV infected individuals have increased B-cell activation and proinflammatory regulatory T-cells",
abstract = "Human T-lymphotropic virus (HTLV) affects the human immune system in many ways, most notably by inducing proliferation of infected CD4 + T cells, but several other cell types are also affected. To characterize the effects of HTLV infection, we analysed blood samples from HTLV-infected individuals by flow cytometry. Samples were collected from visitors at the HIV clinic in Bissau, Guinea-Bissau. These samples were tested for HTLV and HIV, and 199 were analysed by flow cytometry using panels for B cells, T-cell maturation and activation, regulatory T cells (Tregs) and monocytes. CD80+ cell proportions were significantly higher in HTLV infected than in HTLV uninfected in all B cell subsets. Among T cells, there was no change in cell distribution between maturation stages, but a higher CD25+ proportion among Tregs (61.1 % vs 36.3 %, p < 0.001) in HTLV infected than in HTLV uninfected. The level of CD49d on individual cells was also higher (MFI 2734.5 vs 1,041, p < 0.001). In HTLV infected individuals, CD8 + T cells had a lower proportion of CTLA-4+ (2.5 % vs 3.5 %, 0.048) and higher PD1+ proportion on the CD45RO + subset (81.6 % vs 77.1 %, p < 0.001). Together, these findings point toward reduced regulation in HTLV + patients, which leads to immune activation. This study corroborates previous findings and offers new insight into the effects of HTLV by providing a broad flowcytometric analysis of immune cells in HTLV + individuals.",
keywords = "CD49d, CD80, HIV, HTLV, Immune dysregulation, Spectral flow cytometry",
author = "Bertram Kjerulff and Petersen, {Mikkel Steen} and Rodrigues, {Candida Medina} and {da Silva T{\'e}}, David and Mette Christiansen and Christian Erikstrup and H{\o}nge, {Bo Langhoff}",
note = "Copyright {\textcopyright} 2019 Elsevier GmbH. All rights reserved.",
year = "2020",
doi = "10.1016/j.imbio.2019.11.013",
language = "English",
volume = "225",
journal = "Immunobiology",
issn = "0171-2985",
publisher = "Elsevier GmbH - Urban und Fischer",
number = "2",

}

RIS

TY - JOUR

T1 - HTLV infected individuals have increased B-cell activation and proinflammatory regulatory T-cells

AU - Kjerulff, Bertram

AU - Petersen, Mikkel Steen

AU - Rodrigues, Candida Medina

AU - da Silva Té, David

AU - Christiansen, Mette

AU - Erikstrup, Christian

AU - Hønge, Bo Langhoff

N1 - Copyright © 2019 Elsevier GmbH. All rights reserved.

PY - 2020

Y1 - 2020

N2 - Human T-lymphotropic virus (HTLV) affects the human immune system in many ways, most notably by inducing proliferation of infected CD4 + T cells, but several other cell types are also affected. To characterize the effects of HTLV infection, we analysed blood samples from HTLV-infected individuals by flow cytometry. Samples were collected from visitors at the HIV clinic in Bissau, Guinea-Bissau. These samples were tested for HTLV and HIV, and 199 were analysed by flow cytometry using panels for B cells, T-cell maturation and activation, regulatory T cells (Tregs) and monocytes. CD80+ cell proportions were significantly higher in HTLV infected than in HTLV uninfected in all B cell subsets. Among T cells, there was no change in cell distribution between maturation stages, but a higher CD25+ proportion among Tregs (61.1 % vs 36.3 %, p < 0.001) in HTLV infected than in HTLV uninfected. The level of CD49d on individual cells was also higher (MFI 2734.5 vs 1,041, p < 0.001). In HTLV infected individuals, CD8 + T cells had a lower proportion of CTLA-4+ (2.5 % vs 3.5 %, 0.048) and higher PD1+ proportion on the CD45RO + subset (81.6 % vs 77.1 %, p < 0.001). Together, these findings point toward reduced regulation in HTLV + patients, which leads to immune activation. This study corroborates previous findings and offers new insight into the effects of HTLV by providing a broad flowcytometric analysis of immune cells in HTLV + individuals.

AB - Human T-lymphotropic virus (HTLV) affects the human immune system in many ways, most notably by inducing proliferation of infected CD4 + T cells, but several other cell types are also affected. To characterize the effects of HTLV infection, we analysed blood samples from HTLV-infected individuals by flow cytometry. Samples were collected from visitors at the HIV clinic in Bissau, Guinea-Bissau. These samples were tested for HTLV and HIV, and 199 were analysed by flow cytometry using panels for B cells, T-cell maturation and activation, regulatory T cells (Tregs) and monocytes. CD80+ cell proportions were significantly higher in HTLV infected than in HTLV uninfected in all B cell subsets. Among T cells, there was no change in cell distribution between maturation stages, but a higher CD25+ proportion among Tregs (61.1 % vs 36.3 %, p < 0.001) in HTLV infected than in HTLV uninfected. The level of CD49d on individual cells was also higher (MFI 2734.5 vs 1,041, p < 0.001). In HTLV infected individuals, CD8 + T cells had a lower proportion of CTLA-4+ (2.5 % vs 3.5 %, 0.048) and higher PD1+ proportion on the CD45RO + subset (81.6 % vs 77.1 %, p < 0.001). Together, these findings point toward reduced regulation in HTLV + patients, which leads to immune activation. This study corroborates previous findings and offers new insight into the effects of HTLV by providing a broad flowcytometric analysis of immune cells in HTLV + individuals.

KW - CD49d

KW - CD80

KW - HIV

KW - HTLV

KW - Immune dysregulation

KW - Spectral flow cytometry

U2 - 10.1016/j.imbio.2019.11.013

DO - 10.1016/j.imbio.2019.11.013

M3 - Journal article

C2 - 31810824

VL - 225

JO - Immunobiology

JF - Immunobiology

SN - 0171-2985

IS - 2

M1 - 151878

ER -