H2O2 leaf priming improves tolerance to cold stress in pistachio rootstocks

Kiarash Jamshidi Goharrizi; Mohammad Mehdi Momeni; Soraya Karami; Geoffrey Meru; Maryam Nazari; Sedighe Ghanaei; Asma Moeinzadeh

doi:10.1007/s11738-024-03650-y

H₂O₂ leaf priming improves tolerance to cold stress in pistachio rootstocks

Kiarash Jamshidi Goharrizi^*, Mohammad Mehdi Momeni, Soraya Karami, Geoffrey Meru, Maryam Nazari, Sedighe Ghanaei, Asma Moeinzadeh

^*Corresponding author for this work

Department of Agroecology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

2 Citations (Scopus)

Abstract

Hydrogen peroxide (H₂O₂) priming is an effective strategy for alleviating the detrimental effects caused by cold stress in plants, however the underlying functional mechanisms are poorly understood. In the current study, the impact of H₂O₂ priming on the biochemical and physiological processes in pistachio (Pistacia vera L.) rootstocks was determined under cold stress. Overall, across the four rootstocks studied (UCB-1, Badami, Ghazvini and Kaleh-Ghouch), H₂O₂ priming prior to cold stress (primed condition) resulted in an increase in photosynthetic pigments (total chlorophyll and total carotenoid) and total soluble proteins compared to cold stress treatment (unprimed condition). Furthermore, the antioxidant enzymes activity (Ascorbate peroxidase (APX), Polyphenol oxidase (PPO), Catalase (CAT), and Guaiacol peroxidase (GPX)) and osmolyte levels (Proline (PRO), Total free amino acids (TFAA) and Total soluble carbohydrate (TSC)) increased under H₂O₂ priming + cold stress treatment compared to the unprimed condition. On the contrary, oxidative stress parameters (Electrolyte Leakage (EL), Malondialdehyde (MDA), and other aldehydes (OLD)) decreased under primed environment compared to unprimed condition. Overall, H₂O₂ priming improved response to cold stress in pistachio rootstocks; however, there was variations among the genotypes in the physiological and biochemical response. Cold stress in the absence of H₂O₂ priming revealed Badami to be the most tolerant rootstock, followed by Ghazvini, UCB-1 and Kaleh-Ghouch. However, when H₂O₂ priming was applied prior to cold stress exposure, UCB-1 was the most tolerant rootstock, followed by Badami, Ghazvini and Kaleh-Ghouch. Positive correlations were found between antioxidant enzyme activities and osmolytes for UCB-1, Badami and Ghazvini, while conversely, a negative correlation was found between antioxidant enzyme activities and oxidative stress parameters. The outcomes of this study indicate that priming with H₂O₂ can reduce the destructive effects of cold stress on pistachio rootstocks. Thus, H₂O₂ priming may be considered as a valuable strategy for improving the tolerance of cold-sensitive rootstocks, such as UCB-1, and implementing it as a pre-treatment could prevent economic damage resulting from cold stress in agriculture.

Original language	English
Journal	Acta Physiologiae Plantarum
Volume	46
Issue	2
ISSN	0137-5881
DOIs	https://doi.org/10.1007/s11738-024-03650-y
Publication status	Published - Feb 2024

Keywords

Antioxidant enzymes
Cold stress
Hydrogen peroxide priming
Pistachio rootstocks

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10.1007/s11738-024-03650-y

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title = "H2O2 leaf priming improves tolerance to cold stress in pistachio rootstocks",

abstract = "Hydrogen peroxide (H2O2) priming is an effective strategy for alleviating the detrimental effects caused by cold stress in plants, however the underlying functional mechanisms are poorly understood. In the current study, the impact of H2O2 priming on the biochemical and physiological processes in pistachio (Pistacia vera L.) rootstocks was determined under cold stress. Overall, across the four rootstocks studied (UCB-1, Badami, Ghazvini and Kaleh-Ghouch), H2O2 priming prior to cold stress (primed condition) resulted in an increase in photosynthetic pigments (total chlorophyll and total carotenoid) and total soluble proteins compared to cold stress treatment (unprimed condition). Furthermore, the antioxidant enzymes activity (Ascorbate peroxidase (APX), Polyphenol oxidase (PPO), Catalase (CAT), and Guaiacol peroxidase (GPX)) and osmolyte levels (Proline (PRO), Total free amino acids (TFAA) and Total soluble carbohydrate (TSC)) increased under H2O2 priming + cold stress treatment compared to the unprimed condition. On the contrary, oxidative stress parameters (Electrolyte Leakage (EL), Malondialdehyde (MDA), and other aldehydes (OLD)) decreased under primed environment compared to unprimed condition. Overall, H2O2 priming improved response to cold stress in pistachio rootstocks; however, there was variations among the genotypes in the physiological and biochemical response. Cold stress in the absence of H2O2 priming revealed Badami to be the most tolerant rootstock, followed by Ghazvini, UCB-1 and Kaleh-Ghouch. However, when H2O2 priming was applied prior to cold stress exposure, UCB-1 was the most tolerant rootstock, followed by Badami, Ghazvini and Kaleh-Ghouch. Positive correlations were found between antioxidant enzyme activities and osmolytes for UCB-1, Badami and Ghazvini, while conversely, a negative correlation was found between antioxidant enzyme activities and oxidative stress parameters. The outcomes of this study indicate that priming with H2O2 can reduce the destructive effects of cold stress on pistachio rootstocks. Thus, H2O2 priming may be considered as a valuable strategy for improving the tolerance of cold-sensitive rootstocks, such as UCB-1, and implementing it as a pre-treatment could prevent economic damage resulting from cold stress in agriculture.",

keywords = "Antioxidant enzymes, Cold stress, Hydrogen peroxide priming, Pistachio rootstocks",

author = "{Jamshidi Goharrizi}, Kiarash and Momeni, {Mohammad Mehdi} and Soraya Karami and Geoffrey Meru and Maryam Nazari and Sedighe Ghanaei and Asma Moeinzadeh",

note = "Publisher Copyright: {\textcopyright} The Author(s) under exclusive licence to Franciszek G{\'o}rski Institute of Plant Physiology, Polish Academy of Sciences, Krak{\'o}w 2024.",

year = "2024",

month = feb,

doi = "10.1007/s11738-024-03650-y",

language = "English",

volume = "46",

journal = "Acta Physiologiae Plantarum",

issn = "0137-5881",

publisher = "Springer Nature",

number = "2",

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TY - JOUR

T1 - H2O2 leaf priming improves tolerance to cold stress in pistachio rootstocks

AU - Jamshidi Goharrizi, Kiarash

AU - Momeni, Mohammad Mehdi

AU - Karami, Soraya

AU - Meru, Geoffrey

AU - Nazari, Maryam

AU - Ghanaei, Sedighe

AU - Moeinzadeh, Asma

N1 - Publisher Copyright: © The Author(s) under exclusive licence to Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2024.

PY - 2024/2

Y1 - 2024/2

N2 - Hydrogen peroxide (H2O2) priming is an effective strategy for alleviating the detrimental effects caused by cold stress in plants, however the underlying functional mechanisms are poorly understood. In the current study, the impact of H2O2 priming on the biochemical and physiological processes in pistachio (Pistacia vera L.) rootstocks was determined under cold stress. Overall, across the four rootstocks studied (UCB-1, Badami, Ghazvini and Kaleh-Ghouch), H2O2 priming prior to cold stress (primed condition) resulted in an increase in photosynthetic pigments (total chlorophyll and total carotenoid) and total soluble proteins compared to cold stress treatment (unprimed condition). Furthermore, the antioxidant enzymes activity (Ascorbate peroxidase (APX), Polyphenol oxidase (PPO), Catalase (CAT), and Guaiacol peroxidase (GPX)) and osmolyte levels (Proline (PRO), Total free amino acids (TFAA) and Total soluble carbohydrate (TSC)) increased under H2O2 priming + cold stress treatment compared to the unprimed condition. On the contrary, oxidative stress parameters (Electrolyte Leakage (EL), Malondialdehyde (MDA), and other aldehydes (OLD)) decreased under primed environment compared to unprimed condition. Overall, H2O2 priming improved response to cold stress in pistachio rootstocks; however, there was variations among the genotypes in the physiological and biochemical response. Cold stress in the absence of H2O2 priming revealed Badami to be the most tolerant rootstock, followed by Ghazvini, UCB-1 and Kaleh-Ghouch. However, when H2O2 priming was applied prior to cold stress exposure, UCB-1 was the most tolerant rootstock, followed by Badami, Ghazvini and Kaleh-Ghouch. Positive correlations were found between antioxidant enzyme activities and osmolytes for UCB-1, Badami and Ghazvini, while conversely, a negative correlation was found between antioxidant enzyme activities and oxidative stress parameters. The outcomes of this study indicate that priming with H2O2 can reduce the destructive effects of cold stress on pistachio rootstocks. Thus, H2O2 priming may be considered as a valuable strategy for improving the tolerance of cold-sensitive rootstocks, such as UCB-1, and implementing it as a pre-treatment could prevent economic damage resulting from cold stress in agriculture.

AB - Hydrogen peroxide (H2O2) priming is an effective strategy for alleviating the detrimental effects caused by cold stress in plants, however the underlying functional mechanisms are poorly understood. In the current study, the impact of H2O2 priming on the biochemical and physiological processes in pistachio (Pistacia vera L.) rootstocks was determined under cold stress. Overall, across the four rootstocks studied (UCB-1, Badami, Ghazvini and Kaleh-Ghouch), H2O2 priming prior to cold stress (primed condition) resulted in an increase in photosynthetic pigments (total chlorophyll and total carotenoid) and total soluble proteins compared to cold stress treatment (unprimed condition). Furthermore, the antioxidant enzymes activity (Ascorbate peroxidase (APX), Polyphenol oxidase (PPO), Catalase (CAT), and Guaiacol peroxidase (GPX)) and osmolyte levels (Proline (PRO), Total free amino acids (TFAA) and Total soluble carbohydrate (TSC)) increased under H2O2 priming + cold stress treatment compared to the unprimed condition. On the contrary, oxidative stress parameters (Electrolyte Leakage (EL), Malondialdehyde (MDA), and other aldehydes (OLD)) decreased under primed environment compared to unprimed condition. Overall, H2O2 priming improved response to cold stress in pistachio rootstocks; however, there was variations among the genotypes in the physiological and biochemical response. Cold stress in the absence of H2O2 priming revealed Badami to be the most tolerant rootstock, followed by Ghazvini, UCB-1 and Kaleh-Ghouch. However, when H2O2 priming was applied prior to cold stress exposure, UCB-1 was the most tolerant rootstock, followed by Badami, Ghazvini and Kaleh-Ghouch. Positive correlations were found between antioxidant enzyme activities and osmolytes for UCB-1, Badami and Ghazvini, while conversely, a negative correlation was found between antioxidant enzyme activities and oxidative stress parameters. The outcomes of this study indicate that priming with H2O2 can reduce the destructive effects of cold stress on pistachio rootstocks. Thus, H2O2 priming may be considered as a valuable strategy for improving the tolerance of cold-sensitive rootstocks, such as UCB-1, and implementing it as a pre-treatment could prevent economic damage resulting from cold stress in agriculture.

KW - Antioxidant enzymes

KW - Cold stress

KW - Hydrogen peroxide priming

KW - Pistachio rootstocks

UR - http://www.scopus.com/inward/record.url?scp=85184184173&partnerID=8YFLogxK

U2 - 10.1007/s11738-024-03650-y

DO - 10.1007/s11738-024-03650-y

M3 - Journal article

AN - SCOPUS:85184184173

SN - 0137-5881

VL - 46

JO - Acta Physiologiae Plantarum

JF - Acta Physiologiae Plantarum

IS - 2

ER -

H₂O₂ leaf priming improves tolerance to cold stress in pistachio rootstocks

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