How do cable bacteria breathe? Towards identification & characterization of a novel terminal oxidase in cable bacteria

Research output: Contribution to conferencePosterResearch

“Cable bacteria” were discovered in 2012 by researchers at Aarhus
University and revealed a novel type of long-distance electron transport
within their filamentous bodies. While our preliminary data have indicated
that cable bacteria have the highest respiration rate per cell ever
measured, extensive omics data have failed to identify any terminal
oxidase. To shed light on this puzzling observation, we have funneled
down a few candidate redox proteins which could constitute the
respiratory machinery in cable bacteria.
Of prime interest, is a unique multi-domain chimeric protein containing a
multiheme c554 (electron transfer properties) domain flanked by
truncated hemoglobin TrHb (oxygen binding) domains. Large-scale
expression of the individual TrHb domains (D1 and D3) in E. coli followed
by IMAC affinity and gel filtration chromatography has yielded high
amount of pure protein. Expression and purification of the full length
protein has also yielded promising results.
Future studies will involve detailed structural and functional
characterization of the chimeric protein and other redox candidates to
ultimately identify the terminal oxidase enzyme in cable bacteria.
Original languageEnglish
Publication yearNov 2018
Number of pages1
Publication statusPublished - Nov 2018
EventCoLuAa - Aarhus University, Aarhus, Denmark
Duration: 1 Nov 20182 Nov 2018


LocationAarhus University
Internet address

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ID: 141384833