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Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio

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Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio. / Waghmare, Swapnil Gorakh; Samarin, Azin Mohagheghi; Samarin, Azadeh Mohagheghi et al.

In: International Journal of Molecular Sciences (Online), Vol. 22, No. 11, 6036, 06.2021.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Waghmare, SG, Samarin, AM, Samarin, AM, Danielsen, M, Moller, HS, Policar, T, Linhart, O & Dalsgaard, TK 2021, 'Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio', International Journal of Molecular Sciences (Online), vol. 22, no. 11, 6036. https://doi.org/10.3390/ijms22116036

APA

Waghmare, S. G., Samarin, A. M., Samarin, A. M., Danielsen, M., Moller, H. S., Policar, T., Linhart, O., & Dalsgaard, T. K. (2021). Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio. International Journal of Molecular Sciences (Online), 22(11), [6036]. https://doi.org/10.3390/ijms22116036

CBE

Waghmare SG, Samarin AM, Samarin AM, Danielsen M, Moller HS, Policar T, Linhart O, Dalsgaard TK. 2021. Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio. International Journal of Molecular Sciences (Online). 22(11):Article 6036. https://doi.org/10.3390/ijms22116036

MLA

Waghmare, Swapnil Gorakh et al. "Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio". International Journal of Molecular Sciences (Online). 2021. 22(11). https://doi.org/10.3390/ijms22116036

Vancouver

Waghmare SG, Samarin AM, Samarin AM, Danielsen M, Moller HS, Policar T et al. Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio. International Journal of Molecular Sciences (Online). 2021 Jun;22(11):6036. doi: 10.3390/ijms22116036

Author

Waghmare, Swapnil Gorakh ; Samarin, Azin Mohagheghi ; Samarin, Azadeh Mohagheghi et al. / Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio. In: International Journal of Molecular Sciences (Online). 2021 ; Vol. 22, No. 11.

Bibtex

@article{17d9116a7c8940b8acf65aaa9affa84b,
title = "Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio",
abstract = "Aging is the most critical factor that influences the quality of post-ovulatory oocytes. Age-related molecular pathways remain poorly understood in fish oocytes. In this study, we examined the effect of oocyte aging on specific histone acetylation in common carp Cyprinus carpio. The capacity to progress to the larval stage in oocytes that were aged for 28 h in vivo and in vitro was evaluated. Global histone modifications and specific histone acetylation (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) were investigated during oocyte aging. Furthermore, the activity of histone acetyltransferase (HAT) was assessed in fresh and aged oocytes. Global histone modifications did not exhibit significant alterations during 8 h of oocyte aging. Among the selected modifications, H4K12ac increased significantly at 28 h post-stripping (HPS). Although not significantly different, HAT activity exhibited an upward trend during oocyte aging. Results of our current study indicate that aging of common carp oocytes for 12 h results in complete loss of egg viability rates without any consequence in global and specific histone modifications. However, aging oocytes for 28 h led to increased H4K12ac. Thus, histone acetylation modification as a crucial epigenetic mediator may be associated with age-related defects, particularly in oocytes of a more advanced age.",
keywords = "Cyprinus carpio, egg quality, epigenetics, histone acetyltransferase, histone modifications, post-ovulatory aging, EGG QUALITY, GENE-EXPRESSION, RAINBOW-TROUT, EPIGENETIC CHANGES, MESSENGER-RNA, CENP-A, CHROMATIN, PATTERNS, LINNAEUS, LANGUAGE",
author = "Waghmare, {Swapnil Gorakh} and Samarin, {Azin Mohagheghi} and Samarin, {Azadeh Mohagheghi} and Marianne Danielsen and Moller, {Hanne Sondergard} and Tomas Policar and Otomar Linhart and Dalsgaard, {Trine Kastrup}",
year = "2021",
month = jun,
doi = "10.3390/ijms22116036",
language = "English",
volume = "22",
journal = "International Journal of Molecular Sciences ",
issn = "1661-6596",
publisher = "MDPI AG",
number = "11",

}

RIS

TY - JOUR

T1 - Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio

AU - Waghmare, Swapnil Gorakh

AU - Samarin, Azin Mohagheghi

AU - Samarin, Azadeh Mohagheghi

AU - Danielsen, Marianne

AU - Moller, Hanne Sondergard

AU - Policar, Tomas

AU - Linhart, Otomar

AU - Dalsgaard, Trine Kastrup

PY - 2021/6

Y1 - 2021/6

N2 - Aging is the most critical factor that influences the quality of post-ovulatory oocytes. Age-related molecular pathways remain poorly understood in fish oocytes. In this study, we examined the effect of oocyte aging on specific histone acetylation in common carp Cyprinus carpio. The capacity to progress to the larval stage in oocytes that were aged for 28 h in vivo and in vitro was evaluated. Global histone modifications and specific histone acetylation (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) were investigated during oocyte aging. Furthermore, the activity of histone acetyltransferase (HAT) was assessed in fresh and aged oocytes. Global histone modifications did not exhibit significant alterations during 8 h of oocyte aging. Among the selected modifications, H4K12ac increased significantly at 28 h post-stripping (HPS). Although not significantly different, HAT activity exhibited an upward trend during oocyte aging. Results of our current study indicate that aging of common carp oocytes for 12 h results in complete loss of egg viability rates without any consequence in global and specific histone modifications. However, aging oocytes for 28 h led to increased H4K12ac. Thus, histone acetylation modification as a crucial epigenetic mediator may be associated with age-related defects, particularly in oocytes of a more advanced age.

AB - Aging is the most critical factor that influences the quality of post-ovulatory oocytes. Age-related molecular pathways remain poorly understood in fish oocytes. In this study, we examined the effect of oocyte aging on specific histone acetylation in common carp Cyprinus carpio. The capacity to progress to the larval stage in oocytes that were aged for 28 h in vivo and in vitro was evaluated. Global histone modifications and specific histone acetylation (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) were investigated during oocyte aging. Furthermore, the activity of histone acetyltransferase (HAT) was assessed in fresh and aged oocytes. Global histone modifications did not exhibit significant alterations during 8 h of oocyte aging. Among the selected modifications, H4K12ac increased significantly at 28 h post-stripping (HPS). Although not significantly different, HAT activity exhibited an upward trend during oocyte aging. Results of our current study indicate that aging of common carp oocytes for 12 h results in complete loss of egg viability rates without any consequence in global and specific histone modifications. However, aging oocytes for 28 h led to increased H4K12ac. Thus, histone acetylation modification as a crucial epigenetic mediator may be associated with age-related defects, particularly in oocytes of a more advanced age.

KW - Cyprinus carpio

KW - egg quality

KW - epigenetics

KW - histone acetyltransferase

KW - histone modifications

KW - post-ovulatory aging

KW - EGG QUALITY

KW - GENE-EXPRESSION

KW - RAINBOW-TROUT

KW - EPIGENETIC CHANGES

KW - MESSENGER-RNA

KW - CENP-A

KW - CHROMATIN

KW - PATTERNS

KW - LINNAEUS

KW - LANGUAGE

U2 - 10.3390/ijms22116036

DO - 10.3390/ijms22116036

M3 - Journal article

C2 - 34204879

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 11

M1 - 6036

ER -