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Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio

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DOI

  • Swapnil Gorakh Waghmare, Univ South Bohemia Ceske Budejovice, University of South Bohemia Ceske Budejovice, Res Inst Fish Culture & Hydrobiol, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy
  • ,
  • Azin Mohagheghi Samarin, Univ South Bohemia Ceske Budejovice, University of South Bohemia Ceske Budejovice, Res Inst Fish Culture & Hydrobiol, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy
  • ,
  • Azadeh Mohagheghi Samarin, Univ South Bohemia Ceske Budejovice, University of South Bohemia Ceske Budejovice, Res Inst Fish Culture & Hydrobiol, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy
  • ,
  • Marianne Danielsen
  • Hanne Sondergard Moller
  • Tomas Policar, Univ South Bohemia Ceske Budejovice, University of South Bohemia Ceske Budejovice, Res Inst Fish Culture & Hydrobiol, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy
  • ,
  • Otomar Linhart, Univ South Bohemia Ceske Budejovice, University of South Bohemia Ceske Budejovice, Res Inst Fish Culture & Hydrobiol, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy
  • ,
  • Trine Kastrup Dalsgaard

Aging is the most critical factor that influences the quality of post-ovulatory oocytes. Age-related molecular pathways remain poorly understood in fish oocytes. In this study, we examined the effect of oocyte aging on specific histone acetylation in common carp Cyprinus carpio. The capacity to progress to the larval stage in oocytes that were aged for 28 h in vivo and in vitro was evaluated. Global histone modifications and specific histone acetylation (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) were investigated during oocyte aging. Furthermore, the activity of histone acetyltransferase (HAT) was assessed in fresh and aged oocytes. Global histone modifications did not exhibit significant alterations during 8 h of oocyte aging. Among the selected modifications, H4K12ac increased significantly at 28 h post-stripping (HPS). Although not significantly different, HAT activity exhibited an upward trend during oocyte aging. Results of our current study indicate that aging of common carp oocytes for 12 h results in complete loss of egg viability rates without any consequence in global and specific histone modifications. However, aging oocytes for 28 h led to increased H4K12ac. Thus, histone acetylation modification as a crucial epigenetic mediator may be associated with age-related defects, particularly in oocytes of a more advanced age.

Original languageEnglish
Article number6036
JournalInternational Journal of Molecular Sciences (Online)
Volume22
Issue11
Number of pages11
ISSN1661-6596
DOIs
Publication statusPublished - Jun 2021

    Research areas

  • Cyprinus carpio, egg quality, epigenetics, histone acetyltransferase, histone modifications, post-ovulatory aging, EGG QUALITY, GENE-EXPRESSION, RAINBOW-TROUT, EPIGENETIC CHANGES, MESSENGER-RNA, CENP-A, CHROMATIN, PATTERNS, LINNAEUS, LANGUAGE

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