Highly effective mlo-based powdery mildew resistance in hexaploid wheat without pleiotropic effects

Christina R. Ingvardsen; Julio A. Massange-Sánchez; Finn Borum; Winnie S. Füchtbauer; Merethe Bagge; Søren Knudsen; Per L. Gregersen

doi:10.1016/j.plantsci.2023.111785

Highly effective mlo-based powdery mildew resistance in hexaploid wheat without pleiotropic effects

Christina R. Ingvardsen, Julio A. Massange-Sánchez, Finn Borum, Winnie S. Füchtbauer, Merethe Bagge, Søren Knudsen, Per L. Gregersen^*

^*Corresponding author for this work

Department of Agroecology - Crop Genetics and Biotechnology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Application of the mlo-based resistance in barley against powdery mildew attacks is a major success in crop breeding, since it confers durable disease resistance. Resistance caused by mutations in the Mlo gene seems to be ubiquitous across a range of species. This work addresses the introduction of mlo-based resistance into hexaploid wheat, which is complicated by the occurrence of three homoeologous genes: Mlo-A1, Mlo-B1 and Mlo-D1. EMS-generated mutant plants were screened for mutations in the three homoeologues. We selected and combined 6, 8, and 4 mutations, respectively, to obtain triple homozygous mlo mutant lines. Twenty-four mutant lines showed highly effective resistance towards attack by the powdery mildew pathogen under field conditions. All 18 mutations appeared to contribute to resistance; however, they had different effects on the occurrence of symptoms such as chlorotic and necrotic spots, which are pleiotropic to the mlo-based powdery mildew resistance. We conclude that to obtain highly effective powdery mildew resistance in wheat and to avoid detrimental pleiotropic effects, all three Mlo homoeologues should be mutated; however, at least one of the mutations should be of the weaker type in order to alleviate strong pleiotropic effects from the other mutations.

Original language	English
Article number	111785
Journal	Plant Science
Volume	335
ISSN	0168-9452
DOIs	https://doi.org/10.1016/j.plantsci.2023.111785
Publication status	Published - Oct 2023

Keywords

EMS mutagenesis
Hexaploid wheat
Mlo
Pleiotropic effects
Powdery mildew resistance
Triticum aestivum

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10.1016/j.plantsci.2023.111785Licence: CC BY

1-s2.0-S0168945223002029-mainFinal published version, 1.22 MBLicence: CC BY

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title = "Highly effective mlo-based powdery mildew resistance in hexaploid wheat without pleiotropic effects",

abstract = "Application of the mlo-based resistance in barley against powdery mildew attacks is a major success in crop breeding, since it confers durable disease resistance. Resistance caused by mutations in the Mlo gene seems to be ubiquitous across a range of species. This work addresses the introduction of mlo-based resistance into hexaploid wheat, which is complicated by the occurrence of three homoeologous genes: Mlo-A1, Mlo-B1 and Mlo-D1. EMS-generated mutant plants were screened for mutations in the three homoeologues. We selected and combined 6, 8, and 4 mutations, respectively, to obtain triple homozygous mlo mutant lines. Twenty-four mutant lines showed highly effective resistance towards attack by the powdery mildew pathogen under field conditions. All 18 mutations appeared to contribute to resistance; however, they had different effects on the occurrence of symptoms such as chlorotic and necrotic spots, which are pleiotropic to the mlo-based powdery mildew resistance. We conclude that to obtain highly effective powdery mildew resistance in wheat and to avoid detrimental pleiotropic effects, all three Mlo homoeologues should be mutated; however, at least one of the mutations should be of the weaker type in order to alleviate strong pleiotropic effects from the other mutations.",

keywords = "EMS mutagenesis, Hexaploid wheat, Mlo, Pleiotropic effects, Powdery mildew resistance, Triticum aestivum",

author = "Ingvardsen, {Christina R.} and Massange-S{\'a}nchez, {Julio A.} and Finn Borum and F{\"u}chtbauer, {Winnie S.} and Merethe Bagge and S{\o}ren Knudsen and Gregersen, {Per L.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = oct,

doi = "10.1016/j.plantsci.2023.111785",

language = "English",

volume = "335",

journal = "Plant Science",

issn = "0168-9452",

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Highly effective mlo-based powdery mildew resistance in hexaploid wheat without pleiotropic effects. / Ingvardsen, Christina R.; Massange-Sánchez, Julio A.; Borum, Finn et al.
In: Plant Science, Vol. 335, 111785, 10.2023.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Highly effective mlo-based powdery mildew resistance in hexaploid wheat without pleiotropic effects

AU - Ingvardsen, Christina R.

AU - Massange-Sánchez, Julio A.

AU - Borum, Finn

AU - Füchtbauer, Winnie S.

AU - Bagge, Merethe

AU - Knudsen, Søren

AU - Gregersen, Per L.

PY - 2023/10

Y1 - 2023/10

N2 - Application of the mlo-based resistance in barley against powdery mildew attacks is a major success in crop breeding, since it confers durable disease resistance. Resistance caused by mutations in the Mlo gene seems to be ubiquitous across a range of species. This work addresses the introduction of mlo-based resistance into hexaploid wheat, which is complicated by the occurrence of three homoeologous genes: Mlo-A1, Mlo-B1 and Mlo-D1. EMS-generated mutant plants were screened for mutations in the three homoeologues. We selected and combined 6, 8, and 4 mutations, respectively, to obtain triple homozygous mlo mutant lines. Twenty-four mutant lines showed highly effective resistance towards attack by the powdery mildew pathogen under field conditions. All 18 mutations appeared to contribute to resistance; however, they had different effects on the occurrence of symptoms such as chlorotic and necrotic spots, which are pleiotropic to the mlo-based powdery mildew resistance. We conclude that to obtain highly effective powdery mildew resistance in wheat and to avoid detrimental pleiotropic effects, all three Mlo homoeologues should be mutated; however, at least one of the mutations should be of the weaker type in order to alleviate strong pleiotropic effects from the other mutations.

AB - Application of the mlo-based resistance in barley against powdery mildew attacks is a major success in crop breeding, since it confers durable disease resistance. Resistance caused by mutations in the Mlo gene seems to be ubiquitous across a range of species. This work addresses the introduction of mlo-based resistance into hexaploid wheat, which is complicated by the occurrence of three homoeologous genes: Mlo-A1, Mlo-B1 and Mlo-D1. EMS-generated mutant plants were screened for mutations in the three homoeologues. We selected and combined 6, 8, and 4 mutations, respectively, to obtain triple homozygous mlo mutant lines. Twenty-four mutant lines showed highly effective resistance towards attack by the powdery mildew pathogen under field conditions. All 18 mutations appeared to contribute to resistance; however, they had different effects on the occurrence of symptoms such as chlorotic and necrotic spots, which are pleiotropic to the mlo-based powdery mildew resistance. We conclude that to obtain highly effective powdery mildew resistance in wheat and to avoid detrimental pleiotropic effects, all three Mlo homoeologues should be mutated; however, at least one of the mutations should be of the weaker type in order to alleviate strong pleiotropic effects from the other mutations.

KW - EMS mutagenesis

KW - Hexaploid wheat

KW - Mlo

KW - Pleiotropic effects

KW - Powdery mildew resistance

KW - Triticum aestivum

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U2 - 10.1016/j.plantsci.2023.111785

DO - 10.1016/j.plantsci.2023.111785

M3 - Journal article

C2 - 37419327

AN - SCOPUS:85165038840

SN - 0168-9452

VL - 335

JO - Plant Science

JF - Plant Science

M1 - 111785

ER -

Highly effective mlo-based powdery mildew resistance in hexaploid wheat without pleiotropic effects

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