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High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet

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  • Tina Skau Nielsen
  • Zach Bendiks, University of California at Davis, United States
  • Bo Thomsen
  • Matthew E Wright, University of California at Davis, United States
  • Peter Kappel Theil
  • ,
  • Benjamin L Scherer, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia
  • Maria L. Marco, University of California at Davis, United States
High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC. The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)—produced by an organocatalytic route—could
oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC. Rats were fed a HPM diet or an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB, for 4 weeks. Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.
The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism. The HAMSB diet also substantially increased fecal butyrate concentration and the pool, compared with the other diets. All three RS treatments altered the cecal microbial composition in a diet specific manner. HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels. Overall, HAMSB consumption showed the most potential for limiting the negative effects of a high-meat diet.

Original languageEnglish
Article number2137
JournalInternational Journal of Molecular Sciences (Online)
Number of pages19
Publication statusPublished - Apr 2019

    Research areas

  • butyrate, resistant starch, colon, Short-chain fatty acids, microbiome, micro-RNA expression, DNA-adduct, Resistant starch, Colon, Butyrate, Micro-RNA expression, Microbiome

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