Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana. / Hebelstrup, Kim; van Zanten, Martijn; Mandon, Julien; Voesenek, Laurentius A C J; Harren, Frans J M; Cristescu, Simona M; Møller, Ian Max; Mur, Luis A J.

In: Journal of Experimental Botany, Vol. 63, No. 15, 21.08.2012, p. 5581-5591.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Hebelstrup, K, van Zanten, M, Mandon, J, Voesenek, LACJ, Harren, FJM, Cristescu, SM, Møller, IM & Mur, LAJ 2012, 'Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana', Journal of Experimental Botany, vol. 63, no. 15, pp. 5581-5591. https://doi.org/10.1093/jxb/ers210

APA

Hebelstrup, K., van Zanten, M., Mandon, J., Voesenek, L. A. C. J., Harren, F. J. M., Cristescu, S. M., Møller, I. M., & Mur, L. A. J. (2012). Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana. Journal of Experimental Botany, 63(15), 5581-5591. https://doi.org/10.1093/jxb/ers210

CBE

Hebelstrup K, van Zanten M, Mandon J, Voesenek LACJ, Harren FJM, Cristescu SM, Møller IM, Mur LAJ. 2012. Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana. Journal of Experimental Botany. 63(15):5581-5591. https://doi.org/10.1093/jxb/ers210

MLA

Vancouver

Hebelstrup K, van Zanten M, Mandon J, Voesenek LACJ, Harren FJM, Cristescu SM et al. Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana. Journal of Experimental Botany. 2012 Aug 21;63(15):5581-5591. https://doi.org/10.1093/jxb/ers210

Author

Hebelstrup, Kim ; van Zanten, Martijn ; Mandon, Julien ; Voesenek, Laurentius A C J ; Harren, Frans J M ; Cristescu, Simona M ; Møller, Ian Max ; Mur, Luis A J. / Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana. In: Journal of Experimental Botany. 2012 ; Vol. 63, No. 15. pp. 5581-5591.

Bibtex

@article{871b0d84575b4b35831db4e5ee29831b,
title = "Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana",
abstract = "Nitric oxide (NO) and ethylene are signalling molecules that are synthesized in response to oxygen depletion. Non-symbiotic plant haemoglobins (Hbs) have been demonstrated to act in roots under oxygen depletion to scavenge NO. Using Arabidopsis thaliana plants, the online emission of NO or ethylene was directly quantified under normoxia, hypoxia (0.1–1.0% O2), or full anoxia. The production of both gases was increased with reduced expression of either of the Hb genes GLB1 or GLB2, whereas NO emission decreased in plants overexpressing these genes. NO emission in plants with reduced Hb gene expression represented a major loss of nitrogen equivalent to 0.2mM nitrate per 24h under hypoxic conditions. Hb gene expression was greatly enhanced in flooded roots, suggesting induction by reduced oxygen diffusion. The function could be to limit loss of nitrogen under NO emission. NO reacts with thiols to form S-nitrosylated compounds, and it is demonstrated that hypoxia substantially increased the content of S-nitrosylated compounds. A parallel up-regulation of Hb gene expression in the normoxic shoots of the flooded plants may reflect signal transmission from root to shoot via ethylene and a role for Hb in the shoots. Hb gene expression was correlated with ethylene-induced upward leaf movement (hyponastic growth) but not with hypocotyl growth, which was Hb independent. Taken together the data suggest that Hb can influence flood-induced hyponasty via ethylene-dependent and, possibly, ethylene-independent pathways.",
keywords = "Ethylene, flooding, haemoglobin, hyponastic growth, hypoxia, nitric oxide (NO)",
author = "Kim Hebelstrup and {van Zanten}, Martijn and Julien Mandon and Voesenek, {Laurentius A C J} and Harren, {Frans J M} and Cristescu, {Simona M} and M{\o}ller, {Ian Max} and Mur, {Luis A J}",
year = "2012",
month = aug,
day = "21",
doi = "10.1093/jxb/ers210",
language = "English",
volume = "63",
pages = "5581--5591",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "15",

}

RIS

TY - JOUR

T1 - Haemoglobin modulates NO emission and hyponasty under hypoxia-related stress in Arabidopsis thaliana

AU - Hebelstrup, Kim

AU - van Zanten, Martijn

AU - Mandon, Julien

AU - Voesenek, Laurentius A C J

AU - Harren, Frans J M

AU - Cristescu, Simona M

AU - Møller, Ian Max

AU - Mur, Luis A J

PY - 2012/8/21

Y1 - 2012/8/21

N2 - Nitric oxide (NO) and ethylene are signalling molecules that are synthesized in response to oxygen depletion. Non-symbiotic plant haemoglobins (Hbs) have been demonstrated to act in roots under oxygen depletion to scavenge NO. Using Arabidopsis thaliana plants, the online emission of NO or ethylene was directly quantified under normoxia, hypoxia (0.1–1.0% O2), or full anoxia. The production of both gases was increased with reduced expression of either of the Hb genes GLB1 or GLB2, whereas NO emission decreased in plants overexpressing these genes. NO emission in plants with reduced Hb gene expression represented a major loss of nitrogen equivalent to 0.2mM nitrate per 24h under hypoxic conditions. Hb gene expression was greatly enhanced in flooded roots, suggesting induction by reduced oxygen diffusion. The function could be to limit loss of nitrogen under NO emission. NO reacts with thiols to form S-nitrosylated compounds, and it is demonstrated that hypoxia substantially increased the content of S-nitrosylated compounds. A parallel up-regulation of Hb gene expression in the normoxic shoots of the flooded plants may reflect signal transmission from root to shoot via ethylene and a role for Hb in the shoots. Hb gene expression was correlated with ethylene-induced upward leaf movement (hyponastic growth) but not with hypocotyl growth, which was Hb independent. Taken together the data suggest that Hb can influence flood-induced hyponasty via ethylene-dependent and, possibly, ethylene-independent pathways.

AB - Nitric oxide (NO) and ethylene are signalling molecules that are synthesized in response to oxygen depletion. Non-symbiotic plant haemoglobins (Hbs) have been demonstrated to act in roots under oxygen depletion to scavenge NO. Using Arabidopsis thaliana plants, the online emission of NO or ethylene was directly quantified under normoxia, hypoxia (0.1–1.0% O2), or full anoxia. The production of both gases was increased with reduced expression of either of the Hb genes GLB1 or GLB2, whereas NO emission decreased in plants overexpressing these genes. NO emission in plants with reduced Hb gene expression represented a major loss of nitrogen equivalent to 0.2mM nitrate per 24h under hypoxic conditions. Hb gene expression was greatly enhanced in flooded roots, suggesting induction by reduced oxygen diffusion. The function could be to limit loss of nitrogen under NO emission. NO reacts with thiols to form S-nitrosylated compounds, and it is demonstrated that hypoxia substantially increased the content of S-nitrosylated compounds. A parallel up-regulation of Hb gene expression in the normoxic shoots of the flooded plants may reflect signal transmission from root to shoot via ethylene and a role for Hb in the shoots. Hb gene expression was correlated with ethylene-induced upward leaf movement (hyponastic growth) but not with hypocotyl growth, which was Hb independent. Taken together the data suggest that Hb can influence flood-induced hyponasty via ethylene-dependent and, possibly, ethylene-independent pathways.

KW - Ethylene

KW - flooding

KW - haemoglobin

KW - hyponastic growth

KW - hypoxia

KW - nitric oxide (NO)

U2 - 10.1093/jxb/ers210

DO - 10.1093/jxb/ers210

M3 - Journal article

C2 - 22915746

VL - 63

SP - 5581

EP - 5591

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 15

ER -