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Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs

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Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs. / Canibe, Nuria; Poulsen, Henrik Vestergaard; Nørgaard, Jan Værum et al.

In: Journal of Animal Science, Vol. 94, No. 7, supp. 3, 10.2016, p. 382-386.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Vancouver

Canibe N, Poulsen HV, Nørgaard JV, Nielsen B, Cantor MD, Derkx P et al. Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs. Journal of Animal Science. 2016 Oct;94(7, supp. 3):382-386. doi: 10.2527/jas2015-9813

Author

Canibe, Nuria ; Poulsen, Henrik Vestergaard ; Nørgaard, Jan Værum et al. / Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs. In: Journal of Animal Science. 2016 ; Vol. 94, No. 7, supp. 3. pp. 382-386.

Bibtex

@article{f3bdf43971b244359ce6ac40e931f254,

title = "Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs",

abstract = "Dietary supplementation with bacteria that are able to overproduce AA in the digestive tract and expected to have a probiotic effect could be a strategy to supply animals with AA and at the same time to gain benefits from the probiotic effect. Three diets were formulated: 1) a basal diet with a Val:Lys of 0.63:1 (Neg), 2) the Neg diet with added Bacillus subtilis-valine (1.28 × 108 cfu/g feed) (+Bac), and 3) the Neg diet with added L-Val to a Val:Lys of 0.69:1 (+Val). Eighteen gilts (6 on each treatment) with initial weights of ∼15 kg were fed the diets for 23 d before the animals were euthanized and samples from the small intestine were obtained. The number of B. subtilis cfu in digesta was higher in the +Bac group than in the Neg group (P < 0.001). In the epithelium, no B. subtilis cfu were detected in the Neg group, whereas numbers between 3.4 and 4.4 log cfu/g and numerically higher Val and Lys concentrations were measured in the +Bac group. Short-term in vitro incubations of digesta showed a decrease (P ≤ 0.03) in the number of B. subtilis cfu over time for the +Bac group and no difference in the rate of Val production compared to that in the Neg group. In conclusion, more B. subtilis cfu were present in the small intestinal digesta and epithelium of pigs fed the +Bac diet. However, the data indicated that the tested B. subtilis-valine was not able to grow in either digesta or in the epithelium and, thereby, to produce significant amounts of Val at these sites.",

author = "Nuria Canibe and Poulsen, {Henrik Vestergaard} and N{\o}rgaard, {Jan V{\ae}rum} and B. Nielsen and M.D. Cantor and P. Derkx and Poulsen, {Hanne Damgaard} and Karoline Blaabjerg and Jensen, {Bent Borg}",

year = "2016",

month = oct,

doi = "10.2527/jas2015-9813",

language = "English",

volume = "94",

pages = "382--386",

journal = "Journal of Animal Science",

issn = "0021-8812",

publisher = "AMER SOC ANIMAL SCIENCE",

number = "7, supp. 3",

}

RIS

TY - JOUR

T1 - Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs

AU - Canibe, Nuria

AU - Poulsen, Henrik Vestergaard

AU - Nørgaard, Jan Værum

AU - Nielsen, B.

AU - Cantor, M.D.

AU - Derkx, P.

AU - Poulsen, Hanne Damgaard

AU - Blaabjerg, Karoline

AU - Jensen, Bent Borg

PY - 2016/10

Y1 - 2016/10

N2 - Dietary supplementation with bacteria that are able to overproduce AA in the digestive tract and expected to have a probiotic effect could be a strategy to supply animals with AA and at the same time to gain benefits from the probiotic effect. Three diets were formulated: 1) a basal diet with a Val:Lys of 0.63:1 (Neg), 2) the Neg diet with added Bacillus subtilis-valine (1.28 × 108 cfu/g feed) (+Bac), and 3) the Neg diet with added L-Val to a Val:Lys of 0.69:1 (+Val). Eighteen gilts (6 on each treatment) with initial weights of ∼15 kg were fed the diets for 23 d before the animals were euthanized and samples from the small intestine were obtained. The number of B. subtilis cfu in digesta was higher in the +Bac group than in the Neg group (P < 0.001). In the epithelium, no B. subtilis cfu were detected in the Neg group, whereas numbers between 3.4 and 4.4 log cfu/g and numerically higher Val and Lys concentrations were measured in the +Bac group. Short-term in vitro incubations of digesta showed a decrease (P ≤ 0.03) in the number of B. subtilis cfu over time for the +Bac group and no difference in the rate of Val production compared to that in the Neg group. In conclusion, more B. subtilis cfu were present in the small intestinal digesta and epithelium of pigs fed the +Bac diet. However, the data indicated that the tested B. subtilis-valine was not able to grow in either digesta or in the epithelium and, thereby, to produce significant amounts of Val at these sites.

AB - Dietary supplementation with bacteria that are able to overproduce AA in the digestive tract and expected to have a probiotic effect could be a strategy to supply animals with AA and at the same time to gain benefits from the probiotic effect. Three diets were formulated: 1) a basal diet with a Val:Lys of 0.63:1 (Neg), 2) the Neg diet with added Bacillus subtilis-valine (1.28 × 108 cfu/g feed) (+Bac), and 3) the Neg diet with added L-Val to a Val:Lys of 0.69:1 (+Val). Eighteen gilts (6 on each treatment) with initial weights of ∼15 kg were fed the diets for 23 d before the animals were euthanized and samples from the small intestine were obtained. The number of B. subtilis cfu in digesta was higher in the +Bac group than in the Neg group (P < 0.001). In the epithelium, no B. subtilis cfu were detected in the Neg group, whereas numbers between 3.4 and 4.4 log cfu/g and numerically higher Val and Lys concentrations were measured in the +Bac group. Short-term in vitro incubations of digesta showed a decrease (P ≤ 0.03) in the number of B. subtilis cfu over time for the +Bac group and no difference in the rate of Val production compared to that in the Neg group. In conclusion, more B. subtilis cfu were present in the small intestinal digesta and epithelium of pigs fed the +Bac diet. However, the data indicated that the tested B. subtilis-valine was not able to grow in either digesta or in the epithelium and, thereby, to produce significant amounts of Val at these sites.

U2 - 10.2527/jas2015-9813

DO - 10.2527/jas2015-9813

M3 - Journal article

VL - 94

SP - 382

EP - 386

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 7, supp. 3

ER -