Growing blood vessels in space: The Spheroids project

Marcus Krueger; Sascha Kopp; Markus Wehland; Johann Bauer; Sarah Baatout; Marjan Moreels; Marcel Egli; Thomas J. Corydon; Manfred Infanger; Daniela Grimm

Growing blood vessels in space: The Spheroids project

Marcus Krueger^*, Sascha Kopp, Markus Wehland, Johann Bauer, Sarah Baatout, Marjan Moreels, Marcel Egli, Thomas J. Corydon, Manfred Infanger, Daniela Grimm

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Conference article › Research › peer-review

Abstract

Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.

Original language	English
Journal	Proceedings of the International Astronautical Congress, IAC
Volume	2018-October
ISSN	0074-1795
Publication status	Published - 2018
Event	69th International Astronautical Congress: #InvolvingEveryone, IAC 2018 - Bremen, Germany Duration: 1 Oct 2018 → 5 Oct 2018

Conference

Conference	69th International Astronautical Congress: #InvolvingEveryone, IAC 2018
Country/Territory	Germany
City	Bremen
Period	01/10/2018 → 05/10/2018

Keywords

3D growth
Microgravity
Random Positioning Machine
Spaceflight
Spheroids
Tubular structures

Cite this

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title = "Growing blood vessels in space: The Spheroids project",

abstract = "Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.",

keywords = "3D growth, Microgravity, Random Positioning Machine, Spaceflight, Spheroids, Tubular structures",

author = "Marcus Krueger and Sascha Kopp and Markus Wehland and Johann Bauer and Sarah Baatout and Marjan Moreels and Marcel Egli and Corydon, {Thomas J.} and Manfred Infanger and Daniela Grimm",

note = "Publisher Copyright: Copyright {\textcopyright} 2018 by the International Astronautical Federation (IAF). All rights reserved.; 69th International Astronautical Congress: #InvolvingEveryone, IAC 2018 ; Conference date: 01-10-2018 Through 05-10-2018",

year = "2018",

language = "English",

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journal = "Proceedings of the International Astronautical Congress, IAC",

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T2 - 69th International Astronautical Congress: #InvolvingEveryone, IAC 2018

AU - Krueger, Marcus

AU - Kopp, Sascha

AU - Wehland, Markus

AU - Bauer, Johann

AU - Baatout, Sarah

AU - Moreels, Marjan

AU - Egli, Marcel

AU - Corydon, Thomas J.

AU - Infanger, Manfred

AU - Grimm, Daniela

PY - 2018

Y1 - 2018

N2 - Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.

AB - Under normal culture conditions endothelial cells (ECs) grow as single layers on the bottom surface of cell culture flasks. In numerous experiments using simulated microgravity we noticed that the ECs formed three-dimensional, tube-like cell aggregates resembling the intima of small, rudimentary blood vessels. The SPHEROIDS project has now shown that similar processes occur in space. For the first time, we could observe scaffold-free growth of human ECs into multicellular spheroids and tubular structures during an experiment in real microgravity. With further investigation of the space samples we hope to understand endothelial 3D growth and to improve the in vitro engineering of biocompatible vessels which could be used in surgery.

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KW - Microgravity

KW - Random Positioning Machine

KW - Spaceflight

KW - Spheroids

KW - Tubular structures

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M3 - Conference article

AN - SCOPUS:85065340400

SN - 0074-1795

VL - 2018-October

JO - Proceedings of the International Astronautical Congress, IAC

JF - Proceedings of the International Astronautical Congress, IAC

Y2 - 1 October 2018 through 5 October 2018

ER -

Growing blood vessels in space: The Spheroids project

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Keywords

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