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Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis

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  • Z. Cheng, The Royal Veterinary College, United Kingdom
  • Laura Buggiotti, Royal Veterinary College, Hatfield, AL9 7TA, United Kingdom, United Kingdom
  • Mazdak Salavati, Royal Veterinary College, Hatfield, AL9 7TA, United Kingdom, University of Edinburgh, United Kingdom
  • Cinzia Marchitelli, Research Center for Animal Production and Aquaculture (CREA), Italy
  • Sergio Palma-Vera, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany
  • Alistair Wylie, Agri-Food and Biosciences Institute, 18a Newforge Lane, BT9 5PX Belfast, United Kingdom
  • Haruko Takeda, Unit of Animal Genomics, GIGA Institute, University of Liège, B-4000 Liège, Belgium, Belgium
  • Lijing Tang, Unit of Animal Genomics, GIGA Institute, University of Liège, B-4000 Liège, Belgium, Belgium
  • Mark A. Crowe, School of Veterinary Medicine, UCD Conway Institute, University College Dublin, Ireland., Ireland
  • D. Claire Wathes, Royal Veterinary College, Hatfield, AL9 7TA, United Kingdom, United Kingdom
  • GplusE Consortium, Genotype Plus Environment Consortium (www.gpluse.eu)
Bovine mastitis, an inflammatory disease of the mammary gland, is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to combat infection. We compared the transcriptomic profiles in circulating leukocytes between healthy cows and those with naturally occurring subclinical or clinical mastitis. Holstein Friesian dairy cows from six farms in EU countries were recruited. Based on milk somatic cell count and clinical records, cows were classified as healthy (n = 147), subclinically (n = 45) or clinically mastitic (n = 22). Circulating leukocyte RNA was sequenced with Illumina NextSeq single end reads (30 M). Differentially expressed genes (DEGs) between the groups were identified using CLC Genomics Workbench V21, followed by GO enrichment analysis. Both subclinical and clinical mastitis caused significant changes in the leukocyte transcriptome, with more intensive changes attributed to clinical mastitis. We detected 769 DEGs between clinical and healthy groups, 258 DEGs between subclinical and healthy groups and 193 DEGs between clinical and subclinical groups. Most DEGs were associated with cell killing and immune processes. Many upregulated DEGs in clinical mastitis encoded antimicrobial peptides (AZU1, BCL3, CAMP, CATHL1, CATHL2, CATHL4,CATHL5, CATHL6, CCL1, CXCL2, CXCL13, DEFB1, DEFB10, DEFB4A, DEFB7, LCN2, PGLYRP1, PRTN3, PTX3, S100A8, S100A9, S100A12, SLC11A1, TF and LTF) which were not upregulated in subclinical mastitis. The use of transcriptomic profiles has identified a much greater up-regulation of genes encoding antimicrobial peptides in circulating leukocytes of cows with naturally occurring clinical compared with subclinical mastitis. These could play a key role in combatting disease organisms.
Original languageEnglish
JournalMolecular Biology Reports
Volume48
Pages (from-to)4611-4623
Number of pages13
ISSN0301-4851
DOIs
Publication statusPublished - Jun 2021

    Research areas

  • Bovine mastitis, Inflammation, Next generation sequence, Transcriptome, IMMUNE-RESPONSE, INNATE, ANTIMICROBIAL PEPTIDES, HOST-DEFENSE PEPTIDES, DAIRY-COWS, GENE-EXPRESSION, INTRAMAMMARY INFECTION, BOVINE MASTITIS, HEALTH, MAMMARY-GLAND

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