Giant Unilamellar Vesicle Microarrays for Cell Function Study

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  • Chuntao Zhu, Harbin Institute of Technology
  • ,
  • Qingchuan Li, Harbin Institute of Technology
  • ,
  • Mingdong Dong
  • Xiaojun Han, Harbin Institute of Technology

Giant unilamellar vesicles (GUVs) are widely used as artificial cell models which contribute to elucidate fundamental questions on origin of life and cell functions. Herein, the GUV microarrays were developed using a point-to-plane electrode system combined with microcontact stripping technique. The biomolecules (DNA, etc.) were selectively encapsulated only inside patterned GUVs. The GUV arrays were used to investigate species mass transport across cell membranes. The release of carboxyfluorescein from GUVs showed a melittin concentration dependent manner. The diffusion coefficient were 0.37 x 10(-11), 0.36 x 0.54 x 10(-11,) 1.10 x 10(-11), 1.74 x 10(-11), 2.31 X 10(-11), and 3.62 X 10(-11) m(2)/s for 0, 0.5, 1.0, 2.0, 3.0, 4.0, and 5.0 mu M melittin, respectively. The GUV arrays were also a good platform for cell metabolism investigation. Horseradish peroxidase (HRP) loaded GUV microarrays were used to mimic internal metabolism by exposing them to the substrates of H2O2 and o-PD to yield fluorescent 2,3-diaminophenazine (2,3-DAP). The proposed GUV arrays have great potential in cell function studies.

Original languageEnglish
JournalAnalytical Chemistry
Volume90
Issue24
Pages (from-to)14363-14367
Number of pages5
ISSN0003-2700
DOIs
Publication statusPublished - 18 Dec 2018

    Research areas

  • POLYMERSOMES, EXPRESSION, MELITTIN, ARRAYS

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