Genome-Wide Identification and Comparative Analysis of ARF Family Genes in Three Apiaceae Species

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  • Qiaoying Pei, North China University of Science and Technology
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  • Nan Li, College of Life Sciences/Center for Genomics and Bio-computing, North China University of Science and Technology
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  • Qihang Yang, North China University of Science and Technology
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  • Tong Wu, North China University of Science and Technology
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  • Shuyan Feng, North China University of Science and Technology
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  • Xuehuan Feng, University of Nebraska–Lincoln
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  • Zange Jing, Kunming University of Science and Technology
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  • Rong Zhou
  • Ke Gong, North China University of Science and Technology
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  • Tong Yu, North China University of Science and Technology
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  • Zhen Wang, Suzhou Polytechnic Institute of Agriculture
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  • Xiaoming Song, North China University of Science and Technology, University of Nebraska–Lincoln, University of Electronic Science and Technology of China

The family Apiaceae includes many important vegetables and medicinal plants. Auxin response factors (ARFs) play critical roles in regulating plant growth and development. Here, we performed a comprehensive analysis of the ARF gene family in three Apiaceae species, celery, coriander, and carrot, and compared the results with the ARF gene family of lettuce, Arabidopsis, and grape. We identified 156 ARF genes in all six species and 89 genes in the three Apiaceae species, including 28, 34, and 27 in celery, coriander, and carrot, respectively. The paralogous gene number in coriander was far greater than that in carrot and celery. Our analysis revealed that ARF genes of the three Apiaceae species in 34 branches of the phylogenetic tree underwent significant positive selection. Additionally, our findings indicated that whole-genome duplication played an important role in ARF gene family expansion. Coriander contained a greater number of ARF genes than celery and carrot because of more gene duplications and less gene losses. We also analyzed the expression of ARF genes in three tissues by RNA-seq and verified the results by quantitative real-time PCR. Furthermore, we found that several paralogous genes exhibited divergent expression patterns. Overall, this study provides a valuable resource for exploring how ARF family genes regulate plant growth and development in other plants. Since this is the first report of the ARF gene family in Apiaceae, our results will serve as a guide for comparative and functional analyses of ARF and other gene families in Apiaceae.

Original languageEnglish
Article number590535
JournalFrontiers in Genetics
Volume11
Number of pages10
ISSN1664-8021
DOIs
Publication statusPublished - Jan 2021

    Research areas

  • Apiaceae species, ARF gene family, expression pattern, gene duplication and loss, orthologous and paralogous genes, phylogenetic analysis

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