Genome-wide measurement of RNA dissociation from chromatin classifies transcripts by their dynamics and reveals rapid dissociation of enhancer lncRNAs

Evgenia Ntini*, Stefan Budach, Ulf A. Vang Ørom, Annalisa Marsico*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Long non-coding RNAs (lncRNAs) are involved in gene expression regulation in cis. Although enriched in the cell chromatin fraction, to what degree this defines their regulatory potential remains unclear. Furthermore, the factors underlying lncRNA chromatin tethering, as well as the molecular basis of efficient lncRNA chromatin dissociation and its impact on enhancer activity and target gene expression, remain to be resolved. Here, we developed chrTT-seq, which combines the pulse-chase metabolic labeling of nascent RNA with chromatin fractionation and transient transcriptome sequencing to follow nascent RNA transcripts from their transcription on chromatin to release and allows the quantification of dissociation dynamics. By incorporating genomic, transcriptomic, and epigenetic metrics, as well as RNA-binding protein propensities, in machine learning models, we identify features that define transcript groups of different chromatin dissociation dynamics. Notably, lncRNAs transcribed from enhancers display reduced chromatin retention, suggesting that, in addition to splicing, their chromatin dissociation may shape enhancer activity.

Original languageEnglish
JournalCell systems
Volume14
Issue10
Pages (from-to)906-922.e6
ISSN2405-4712
DOIs
Publication statusPublished - Oct 2023

Keywords

  • chromatin dissociation dynamics
  • co-transcriptional splicing
  • enhancer
  • enhancer-associated lncRNAs
  • lncRNAs
  • machine learning
  • nascent RNA transcription
  • predictive models
  • RNA processing
  • RNA-binding protein interactions

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