Genome-wide association for metabolic clusters in early-lactation Holstein dairy cows

H. Atashi, Mazdak Salavati, Jenne De Koster, Mark Crowe, Gert Opsomer, GplusE Consortium, Miel Hostens*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

The aim of this study was to detect the genomic region or regions associated with metabolic clusters in early-lactation Holstein cows. This study was car-ried out in 2 experiments. In experiment I, which was carried out on 105 multiparous Holstein cows, animals were classified through k-means clustering on log-transformed and standardized concentrations of blood glucose, insulin-like growth factor I, free fatty acids, and β-hydroxybutyrate at 14 and 35 d in milk (DIM), into metabolic clusters, either balanced (BAL) or other (OTR). Forty percent of the animals were categorized in the BAL group, and the remainder were categorized as OTR. The cows were genotyped for a total of 777,962 SNP. A genome-wide association study was performed, using a case-control approach through the GEMMA software, accounting for population structure. We found 8 SNP (BTA11, BTA23, and BTAX) associated with the predicted metabolic clusters. In experiment II, carried out on 4,267 second-parity Holstein cows, milk samples collected starting from the first week un-til 50 DIM were used to determine Fourier-transform mid-infrared (FT-MIR) spectra and subsequently to classify the animals into the same metabolic clusters (BAL vs. OTR). Twenty-eight percent of the animals were categorized in the BAL group, and the remainder were classified in the OTR category. Although daily milk yield was lower in BAL cows, we found no differ-ence in daily fat- and protein-corrected milk yield in cows from the BAL metabolic cluster compared with those in the OTR metabolic cluster. In the next step, a single-step genomic BLUP was used to identify the genomic region(s) associated with the predicted metabolic clusters. The results revealed that prediction of metabolic clusters is a highly polygenic trait regulated by many small-sized effects. The region of 36,258 to 36,295 kb on BTA27 was the highly associated region for the predicted metabolic clusters, with the closest genes to this region (ANK1 and miR-486) being related to hematopoiesis, erythropoiesis, and mammary gland development. The heritability for metabolic clustering was 0.17 (SD 0.03), indicating that the use of FT-MIR spectra in milk to predict metabolic clusters in early-lactation across a large number of cows has satisfactory potential to be included in genetic selection programs for modern dairy cows.
Original languageEnglish
JournalJournal of Dairy Science
Volume103
Issue7
Pages (from-to)6392-6406
Number of pages15
ISSN0022-0302
DOIs
Publication statusPublished - Jul 2020

Keywords

  • metabolic adaptation
  • transition period
  • genome-wide association study
  • dairy cow
  • Lactation/physiology
  • Genome-Wide Association Study
  • Blood Glucose/metabolism
  • Fatty Acids, Nonesterified/blood
  • Milk/chemistry
  • Case-Control Studies
  • Pregnancy
  • Cattle/metabolism
  • Animals
  • Female
  • Gene Expression Regulation/physiology
  • Milk Proteins/analysis
  • 3-Hydroxybutyric Acid/blood
  • Cluster Analysis
  • CATTLE
  • HEALTH TRAITS
  • QUANTITATIVE TRAIT LOCI
  • GENETIC-PARAMETERS
  • FEED-EFFICIENCY
  • MILK-PRODUCTION
  • NEGATIVE-ENERGY BALANCE
  • ADAPTATIONS
  • DISEASES
  • ANKYRIN-1 GENE

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