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Genetic suppressors of the Lotus japonicus har1-1 hypernodulation phenotype.

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  • Jeremy Murray, Denmark
  • Bogumil Karas, Denmark
  • Loretta Ross, Denmark
  • Andreas Brachmann, Denmark
  • Cameron Wagg, Denmark
  • Ryan Geil, Denmark
  • Jillian Perry, Denmark
  • Katarzyna Nowakowski, Denmark
  • Mandy MacGillivary, Denmark
  • Mark Held, Denmark
  • Jens Stougaard
  • Larry Peterson, Denmark
  • Martin Parniske, Denmark
  • Krzysztof Szczyglowski, Denmark
  • Department of Molecular Biology
Lotus japonicus har1 mutants respond to inoculation with Mesorhizobium loti by forming an excessive number of nodules due to genetic lesions in the HAR1 autoregulatory receptor kinase gene. In order to expand the repertoire of mutants available for the genetic dissection of the root nodule symbiosis (RNS), a screen for suppressors of the L. japonicus har1-1 hypernodulation phenotype was performed. Of 150,000 M2 plants analyzed, 61 stable L. japonicus double-mutant lines were isolated. In the context of the har1-1 mutation, 26 mutant lines were unable to form RNS, whereas the remaining 35 mutant lines carried more subtle symbiotic phenotypes, either forming white ineffective nodules or showing reduced nodulation capacity. When challenged with Glomus intraradices, 18 of the 61 suppressor lines were unable to establish a symbiosis with this arbuscular mycorrhiza fungus. Using a combined approach of genetic mapping, targeting induced local lesions in genomics, and sequencing, all non-nodulating mutant lines were characterized and shown to represent new alleles of at least nine independent symbiotic loci. The class of mutants with reduced nodulation capacity was of particular interest because some of them may specify novel plant functions that regulate nodule development in L. japonicus. To facilitate mapping of the latter class of mutants, an introgression line, in which the har1-1 allele was introduced into a polymorphic background of L. japonicus ecotype MG20, was constructed.
Udgivelsesdato: 2006-Oct
Original languageEnglish
JournalMolecular Plant - Microbe Interactions
Volume19
Issue10
Pages (from-to)1082-91
Number of pages9
ISSN0894-0282
DOIs
Publication statusPublished - 2006

    Research areas

  • Alleles, Alphaproteobacteria, Chromosome Mapping, Genetic Markers, Lotus, Microsatellite Repeats, Mutation, Mycorrhizae, Phenotype, Phosphotransferases, Plant Proteins, Plant Roots, Sequence Analysis, DNA, Suppression, Genetic, Symbiosis

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