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Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM)
Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
DOI
- https://doi.org/10.1016/j.scr.2016.10.011
Final published version
- Kai Xiong, University of Copenhagen ,
- Yan Zhou
- Poul Hyttel
- Lars Bolund
- Kristine Karla Freude, University of Copenhagen ,
- Yonglun Luo
Human fibroblasts were engineered to express the CRISPR-based synergistic activation mediator (SAM) complex: dCas9-VP64 and MS2-P65-HSF1. Two induced pluripotent stem cells (iPSCs) clones expressing SAM were established by transducing these fibroblasts with lentivirus expressing OCT4, SOX2, KLF4 and C-MYC. We have validated that the reprogramming cassette is silenced in the SAM iPSC clones. Expression of pluripotency genes (OCT4, SOX2, LIN28A, NANOG, GDF3, SSEA4, and TRA-1-60), differentiation potential to all three germ layers, and normal karyotypes are validated. These SAM-iPSCs provide a novel, useful tool to investigate genetic regulation of stem cell proliferation and differentiation through CRISPR-mediated activation of endogenous genes.
| Original language | English |
|---|---|
| Journal | Stem Cell Research |
| Volume | 17 |
| Issue | 3 |
| Pages (from-to) | 665-669 |
| Number of pages | 5 |
| ISSN | 1873-5061 |
| DOIs | |
| Publication status | Published - 17 Nov 2016 |
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ID: 107150649