Generation of a human allergic mast cell phenotype from CD133(+) stem cells

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Generation of a human allergic mast cell phenotype from CD133(+) stem cells. / Hoffmann, Hans Jürgen.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1192, 2014, p. 57-62.

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Hoffmann, Hans Jürgen. / Generation of a human allergic mast cell phenotype from CD133(+) stem cells. In: Methods in molecular biology (Clifton, N.J.). 2014 ; Vol. 1192. pp. 57-62.

Bibtex

@article{caf4c0f3d3d3450d91b0b9c1654efc1d,
title = "Generation of a human allergic mast cell phenotype from CD133(+) stem cells",
abstract = "Cultured human mast cells are a useful tool for research into innate immune responses as well as allergic mechanisms. Mast cells cultured from peripheral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450 mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phenotype of the mast cells.",
author = "Hoffmann, {Hans J{\"u}rgen}",
year = "2014",
doi = "10.1007/978-1-4939-1173-8_4",
language = "English",
volume = "1192",
pages = "57--62",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Generation of a human allergic mast cell phenotype from CD133(+) stem cells

AU - Hoffmann, Hans Jürgen

PY - 2014

Y1 - 2014

N2 - Cultured human mast cells are a useful tool for research into innate immune responses as well as allergic mechanisms. Mast cells cultured from peripheral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450 mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phenotype of the mast cells.

AB - Cultured human mast cells are a useful tool for research into innate immune responses as well as allergic mechanisms. Mast cells cultured from peripheral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450 mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phenotype of the mast cells.

U2 - 10.1007/978-1-4939-1173-8_4

DO - 10.1007/978-1-4939-1173-8_4

M3 - Journal article

VL - 1192

SP - 57

EP - 62

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -