GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

Carsten Suhr Jacobsen; Louise Feld; Mathis Hjort Hjelmsø; Morten Schostag Nielsen; Regin Rønn; Flemming Ekelund; Anders Johnsen; J G Sørensen; Paul Henning Krogh

GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

Carsten Suhr Jacobsen, Louise Feld, Mathis Hjort Hjelmsø, Morten Schostag Nielsen, Regin Rønn, Flemming Ekelund, Anders Johnsen, J G Sørensen, Paul Henning Krogh

Research output: Working paper/Preprint › Working paper › Research › peer-review

Abstract

The project focussed on validating RNA based methods as potential genomic tools in assessment of agricultural soil ecosystems. It was shown that the mRNA based technique was very sensitive and the effects was seen in the same situations as when the OECD nitrification assay showed an effect. 16S rRNA based pyrosequencing of bacterial communities in soil was shown to report different than just DNA based analysis and indicated unlike the DNA measurement that the community was developing. Finally microarray analysis was compared to traditional test for toxicity testing of Folsomia candida and showed a more sensitive response to the pesticide exposure.

Original language	English
Publisher	Bekæmpelsesmiddelforskning fra Miljøstyrelsen
Pages	1-53
Number of pages	53
ISBN (Print)	978-87-93283-27-5
Publication status	Published - 23 Aug 2016

Cite this

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title = "GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem",

abstract = "The project focussed on validating RNA based methods as potential genomic tools in assessment of agricultural soil ecosystems. It was shown that the mRNA based technique was very sensitive and the effects was seen in the same situations as when the OECD nitrification assay showed an effect. 16S rRNA based pyrosequencing of bacterial communities in soil was shown to report different than just DNA based analysis and indicated unlike the DNA measurement that the community was developing. Finally microarray analysis was compared to traditional test for toxicity testing of Folsomia candida and showed a more sensitive response to the pesticide exposure.",

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T1 - GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

AU - Jacobsen, Carsten Suhr

AU - Feld, Louise

AU - Hjelmsø, Mathis Hjort

AU - Nielsen, Morten Schostag

AU - Rønn, Regin

AU - Ekelund, Flemming

AU - Johnsen, Anders

AU - Sørensen, J G

AU - Krogh, Paul Henning

PY - 2016/8/23

Y1 - 2016/8/23

N2 - The project focussed on validating RNA based methods as potential genomic tools in assessment of agricultural soil ecosystems. It was shown that the mRNA based technique was very sensitive and the effects was seen in the same situations as when the OECD nitrification assay showed an effect. 16S rRNA based pyrosequencing of bacterial communities in soil was shown to report different than just DNA based analysis and indicated unlike the DNA measurement that the community was developing. Finally microarray analysis was compared to traditional test for toxicity testing of Folsomia candida and showed a more sensitive response to the pesticide exposure.

AB - The project focussed on validating RNA based methods as potential genomic tools in assessment of agricultural soil ecosystems. It was shown that the mRNA based technique was very sensitive and the effects was seen in the same situations as when the OECD nitrification assay showed an effect. 16S rRNA based pyrosequencing of bacterial communities in soil was shown to report different than just DNA based analysis and indicated unlike the DNA measurement that the community was developing. Finally microarray analysis was compared to traditional test for toxicity testing of Folsomia candida and showed a more sensitive response to the pesticide exposure.

UR - http://www2.mst.dk/Udgiv/publications/2016/06/978-87-93283-27-5.pdf

M3 - Working paper

SN - 978-87-93283-27-5

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EP - 53

BT - GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

PB - Bekæmpelsesmiddelforskning fra Miljøstyrelsen

ER -

GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

Abstract

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Fingerprint

GENEPEASE: GENEPEASE: GENomic tools for assEssment of Pesticide Effects on the Agricultural Soil Ecosystem

Cite this

GENEPEASE Genomic tools for assessment of pesticide effects on the agricultural soil ecosystem

Abstract

Other files and links

Fingerprint

Projects

GENEPEASE: GENEPEASE: GENomic tools for assEssment of Pesticide Effects on the Agricultural Soil Ecosystem

Cite this