Gene expression in the liver remnant is significantly affected by the size of partial hepatectomy: An experimental rat study

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Gene expression in the liver remnant is significantly affected by the size of partial hepatectomy : An experimental rat study. / Meier, Michelle; Knudsen, Anders Riegels; Andersen, Kasper Jarlhelt et al.

In: Gene Expression, Vol. 17, No. 4, 2017, p. 289-299.

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@article{0621189c6d9f4cd5a9ee6d1d1afedb1c,
title = "Gene expression in the liver remnant is significantly affected by the size of partial hepatectomy: An experimental rat study",
abstract = "Extended hepatectomies may result in posthepatectomy liver failure, a condition with a high mortality. The main purpose of the present study was to investigate and compare the gene expression profiles in rats subjected to increasing size of partial hepatectomy (PH). Thirty Wistar rats were subjected to 30%, 70%, or 90% PH, sham operation, or no operation. Twenty-four hours following resection, liver tissue was harvested and genome-wide expression analysis was performed. Cluster analysis revealed two main groupings, one containing the PH(90%) and one containing the remaining groups [baseline, sham, PH(30%), and PH(70%)]. Categorization of specific affected molecular pathways in the PH(90%) group revealed a downregulation of cellular homeostatic function degradation and biosynthesis, whereas proliferation, cell growth, and cellular stress and injury were upregulated in the PH(90%) group. After PH(90%), the main upregulated pathways were mTOR and ILK. The main activated upstream regulators were hepatocyte growth factor and transforming growth factor. With decreasing size of the future liver remnant, the liver tended to prioritize expression of genes involved in cell proliferation and differentiation at the expense of genes involved in metabolism and body homeostasis. This prioritizing may be an essential molecular explanation for posthepatectomy liver failure.",
keywords = "Gene expression, Hepatectomy, Liver failure, Microarray analysis, Regeneration",
author = "Michelle Meier and Knudsen, {Anders Riegels} and Andersen, {Kasper Jarlhelt} and Bjerregaard, {Niels Christian} and Jensen, {Uffe Birk} and Mortensen, {Frank Viborg}",
year = "2017",
doi = "10.3727/105221617X695825",
language = "English",
volume = "17",
pages = "289--299",
journal = "Gene Expression Patterns",
issn = "1567-133X",
publisher = "Elsevier BV",
number = "4",

}

RIS

TY - JOUR

T1 - Gene expression in the liver remnant is significantly affected by the size of partial hepatectomy

T2 - An experimental rat study

AU - Meier, Michelle

AU - Knudsen, Anders Riegels

AU - Andersen, Kasper Jarlhelt

AU - Bjerregaard, Niels Christian

AU - Jensen, Uffe Birk

AU - Mortensen, Frank Viborg

PY - 2017

Y1 - 2017

N2 - Extended hepatectomies may result in posthepatectomy liver failure, a condition with a high mortality. The main purpose of the present study was to investigate and compare the gene expression profiles in rats subjected to increasing size of partial hepatectomy (PH). Thirty Wistar rats were subjected to 30%, 70%, or 90% PH, sham operation, or no operation. Twenty-four hours following resection, liver tissue was harvested and genome-wide expression analysis was performed. Cluster analysis revealed two main groupings, one containing the PH(90%) and one containing the remaining groups [baseline, sham, PH(30%), and PH(70%)]. Categorization of specific affected molecular pathways in the PH(90%) group revealed a downregulation of cellular homeostatic function degradation and biosynthesis, whereas proliferation, cell growth, and cellular stress and injury were upregulated in the PH(90%) group. After PH(90%), the main upregulated pathways were mTOR and ILK. The main activated upstream regulators were hepatocyte growth factor and transforming growth factor. With decreasing size of the future liver remnant, the liver tended to prioritize expression of genes involved in cell proliferation and differentiation at the expense of genes involved in metabolism and body homeostasis. This prioritizing may be an essential molecular explanation for posthepatectomy liver failure.

AB - Extended hepatectomies may result in posthepatectomy liver failure, a condition with a high mortality. The main purpose of the present study was to investigate and compare the gene expression profiles in rats subjected to increasing size of partial hepatectomy (PH). Thirty Wistar rats were subjected to 30%, 70%, or 90% PH, sham operation, or no operation. Twenty-four hours following resection, liver tissue was harvested and genome-wide expression analysis was performed. Cluster analysis revealed two main groupings, one containing the PH(90%) and one containing the remaining groups [baseline, sham, PH(30%), and PH(70%)]. Categorization of specific affected molecular pathways in the PH(90%) group revealed a downregulation of cellular homeostatic function degradation and biosynthesis, whereas proliferation, cell growth, and cellular stress and injury were upregulated in the PH(90%) group. After PH(90%), the main upregulated pathways were mTOR and ILK. The main activated upstream regulators were hepatocyte growth factor and transforming growth factor. With decreasing size of the future liver remnant, the liver tended to prioritize expression of genes involved in cell proliferation and differentiation at the expense of genes involved in metabolism and body homeostasis. This prioritizing may be an essential molecular explanation for posthepatectomy liver failure.

KW - Gene expression

KW - Hepatectomy

KW - Liver failure

KW - Microarray analysis

KW - Regeneration

UR - http://www.scopus.com/inward/record.url?scp=85035777357&partnerID=8YFLogxK

U2 - 10.3727/105221617X695825

DO - 10.3727/105221617X695825

M3 - Journal article

C2 - 28488569

AN - SCOPUS:85035777357

VL - 17

SP - 289

EP - 299

JO - Gene Expression Patterns

JF - Gene Expression Patterns

SN - 1567-133X

IS - 4

ER -