Aarhus University Seal / Aarhus Universitets segl

Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness. / Zeng, Guanghong; Vad, Brian S; Dueholm, Morten S; Christiansen, Gunna; Nilsson, Martin; Tolker-Nielsen, Tim; Nielsen, Per H; Meyer, Rikke L; Otzen, Daniel E.

In: Frontiers in Microbiology, Vol. 6, 07.10.2015, p. 1099.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Zeng, G, Vad, BS, Dueholm, MS, Christiansen, G, Nilsson, M, Tolker-Nielsen, T, Nielsen, PH, Meyer, RL & Otzen, DE 2015, 'Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness', Frontiers in Microbiology, vol. 6, pp. 1099. https://doi.org/10.3389/fmicb.2015.01099

APA

Zeng, G., Vad, B. S., Dueholm, M. S., Christiansen, G., Nilsson, M., Tolker-Nielsen, T., Nielsen, P. H., Meyer, R. L., & Otzen, D. E. (2015). Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness. Frontiers in Microbiology, 6, 1099. https://doi.org/10.3389/fmicb.2015.01099

CBE

Zeng G, Vad BS, Dueholm MS, Christiansen G, Nilsson M, Tolker-Nielsen T, Nielsen PH, Meyer RL, Otzen DE. 2015. Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness. Frontiers in Microbiology. 6:1099. https://doi.org/10.3389/fmicb.2015.01099

MLA

Vancouver

Zeng G, Vad BS, Dueholm MS, Christiansen G, Nilsson M, Tolker-Nielsen T et al. Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness. Frontiers in Microbiology. 2015 Oct 7;6:1099. https://doi.org/10.3389/fmicb.2015.01099

Author

Zeng, Guanghong ; Vad, Brian S ; Dueholm, Morten S ; Christiansen, Gunna ; Nilsson, Martin ; Tolker-Nielsen, Tim ; Nielsen, Per H ; Meyer, Rikke L ; Otzen, Daniel E. / Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness. In: Frontiers in Microbiology. 2015 ; Vol. 6. pp. 1099.

Bibtex

@article{863fe646504547cdbc90ac6baffba5ac,
title = "Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness",
abstract = "The success of Pseudomonas species as opportunistic pathogens derives in great part from their ability to form stable biofilms that offer protection against chemical and mechanical attack. The extracellular matrix of biofilms contains numerous biomolecules, and it has recently been discovered that in Pseudomonas one of the components includes β-sheet rich amyloid fibrils (functional amyloid) produced by the fap operon. However, the role of the functional amyloid within the biofilm has not yet been investigated in detail. Here we investigate how the fap-based amyloid produced by Pseudomonas affects biofilm hydrophobicity and mechanical properties. Using atomic force microscopy imaging and force spectroscopy, we show that the amyloid renders individual cells more resistant to drying and alters their interactions with hydrophobic probes. Importantly, amyloid makes Pseudomonas more hydrophobic and increases biofilm stiffness 20-fold. Deletion of any one of the individual members of in the fap operon (except the putative chaperone FapA) abolishes this ability to increase biofilm stiffness and correlates with the loss of amyloid. We conclude that amyloid makes major contributions to biofilm mechanical robustness.",
author = "Guanghong Zeng and Vad, {Brian S} and Dueholm, {Morten S} and Gunna Christiansen and Martin Nilsson and Tim Tolker-Nielsen and Nielsen, {Per H} and Meyer, {Rikke L} and Otzen, {Daniel E}",
year = "2015",
month = oct,
day = "7",
doi = "10.3389/fmicb.2015.01099",
language = "English",
volume = "6",
pages = "1099",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness

AU - Zeng, Guanghong

AU - Vad, Brian S

AU - Dueholm, Morten S

AU - Christiansen, Gunna

AU - Nilsson, Martin

AU - Tolker-Nielsen, Tim

AU - Nielsen, Per H

AU - Meyer, Rikke L

AU - Otzen, Daniel E

PY - 2015/10/7

Y1 - 2015/10/7

N2 - The success of Pseudomonas species as opportunistic pathogens derives in great part from their ability to form stable biofilms that offer protection against chemical and mechanical attack. The extracellular matrix of biofilms contains numerous biomolecules, and it has recently been discovered that in Pseudomonas one of the components includes β-sheet rich amyloid fibrils (functional amyloid) produced by the fap operon. However, the role of the functional amyloid within the biofilm has not yet been investigated in detail. Here we investigate how the fap-based amyloid produced by Pseudomonas affects biofilm hydrophobicity and mechanical properties. Using atomic force microscopy imaging and force spectroscopy, we show that the amyloid renders individual cells more resistant to drying and alters their interactions with hydrophobic probes. Importantly, amyloid makes Pseudomonas more hydrophobic and increases biofilm stiffness 20-fold. Deletion of any one of the individual members of in the fap operon (except the putative chaperone FapA) abolishes this ability to increase biofilm stiffness and correlates with the loss of amyloid. We conclude that amyloid makes major contributions to biofilm mechanical robustness.

AB - The success of Pseudomonas species as opportunistic pathogens derives in great part from their ability to form stable biofilms that offer protection against chemical and mechanical attack. The extracellular matrix of biofilms contains numerous biomolecules, and it has recently been discovered that in Pseudomonas one of the components includes β-sheet rich amyloid fibrils (functional amyloid) produced by the fap operon. However, the role of the functional amyloid within the biofilm has not yet been investigated in detail. Here we investigate how the fap-based amyloid produced by Pseudomonas affects biofilm hydrophobicity and mechanical properties. Using atomic force microscopy imaging and force spectroscopy, we show that the amyloid renders individual cells more resistant to drying and alters their interactions with hydrophobic probes. Importantly, amyloid makes Pseudomonas more hydrophobic and increases biofilm stiffness 20-fold. Deletion of any one of the individual members of in the fap operon (except the putative chaperone FapA) abolishes this ability to increase biofilm stiffness and correlates with the loss of amyloid. We conclude that amyloid makes major contributions to biofilm mechanical robustness.

U2 - 10.3389/fmicb.2015.01099

DO - 10.3389/fmicb.2015.01099

M3 - Journal article

C2 - 26500638

VL - 6

SP - 1099

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -