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Formation of the alpha 1-microglobulin chromophore in mammalian and insect cells: a novel post-translational mechanism?

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  • Interdisciplinary Nanoscience Center
  • Department of Molecular Biology
alpha 1-Microglobulin is an immunosuppressive plasma protein synthesized by the liver. The isolated protein is yellow-brown, but the hypothetical chromophore has not yet been identified. In this work, it is shown that a human liver cell line, HepG2, grown in a completely synthetic and serum-free medium, secretes alpha 1-microglobulin which is also yellow-brown, suggesting a de novo synthesis of the chromophore by the cells. alpha 1-Microglobulin isolated from the culture medium of insect cells transfected with the gene for rat alpha 1-microglobulin is also yellow-brown, suggesting that the gene carries information about the chromophore. Reduction and alkylation or removal of N- or O-linked carbohydrates by glycosidase treatment did not reduce the colour intensity of the protein. An internal dodecapeptide (amino acid positions 70-81 in human alpha 1-microglobulin) was also yellow-brown. The latter results indicate that the chromophore is linked to the polypeptide. In conclusion, the results suggest that the alpha 1-microglobulin gene carries information activating a post-translational protein modification mechanism which is present in mammalian and insect cells.
Original languageEnglish
JournalF E B S Letters
Pages (from-to)50-4
Number of pages4
Publication statusPublished - 1995

    Research areas

  • Alkylation, Alpha-Globulins, Amino Acid Sequence, Animals, Cell Line, Glycosylation, Humans, Molecular Sequence Data, Moths, Oxidation-Reduction, Peptides, Pigments, Biological, Protein Processing, Post-Translational, Tumor Cells, Cultured

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