Fluorescence peak integration ratio IC:IT as a new potential indicator tracing the compositional changes in chromophoric dissolved organic matter

Yongqiang  Zhou; Kun Shi; Yunlin Zhang; Erik Jeppesen; Xiaohan Liu; Qichao Zhou; Huawu Wu; Xiangming Tang; Guangwei Zhu

doi:10.1016/j.scitotenv.2016.08.196

Fluorescence peak integration ratio IC:IT as a new potential indicator tracing the compositional changes in chromophoric dissolved organic matter

Yongqiang Zhou, Kun Shi, Yunlin Zhang, Erik Jeppesen, Xiaohan Liu, Qichao Zhou, Huawu Wu, Xiangming Tang, Guangwei Zhu

Arctic Research Centre - Arctic Research Center, ECOS Water

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

80 Citations (Scopus)

Abstract

The present study demonstrates that the ratio of fluorescence integration of peak C to peak T (I _C:I _T) can be used as an indicator tracing the compositional dynamics of chromophoric dissolved organic matter (CDOM). CDOM absorption and fluorescence spectroscopy and stable isotope δ ¹³C were determined on a seasonal basis in seventeen Chinese inland waters as well as in a series of mixing and photodegradation experiments in the lab. A strong positive linear correlation was recorded between I _C:I _T and the ratio of terrestrial humic-like C1 to tryptophan-like C4 (C1:C4) derived by parallel factor analysis. The r ² for the linear fitting between I _C:I _T and C1:C4 (r ² = 0.80) was notably higher than between C1:C4 and other indices tested, including the ratio of CDOM absorption at 250 nm to 365 nm, i.e. a(250):a(365) (r ² = 0.09), spectral slope (S _275–295) (r ² = 0.26), spectral slope ratio (S _R) (r ² = 0.31), the humification index (HIX) (r ² = 0.47), the recent autochthonous biological contribution index (BIX) (r ² = 0.27), and a fluorescence index (FI ₃₇₀) (r ² = 0.07). I _C:I _T exhibited larger variability than the remaining six indices and a closer correlation with stable isotope δ ¹³C than that observed for a(250):a(365), S _275–295, S _R, FI ₃₇₀, and BIX during field campaigns. Confirming our field observations, significant correlations were recorded between I _C:I _T and the remaining six indices, and I _C:I _T also demonstrated notably larger variability than the six other indices during our wastewater addition experiment. Compared with HIX, eutrophic water addition and photobleaching substantially decreased I _C:I _T but had no pronounced effect on a(250):a(365), S _275–297, S _R, FI ₃₇₀, and BIX, further suggesting that I _C:I _T is the most efficient indicator of the CDOM compositional dynamics.

Original language	English
Journal	Science of the Total Environment
Volume	574
Pages (from-to)	1588-1598
Number of pages	11
ISSN	0048-9697
DOIs	https://doi.org/10.1016/j.scitotenv.2016.08.196
Publication status	Published - 2016

Keywords

Chromophoric dissolved organic matter (CDOM)
Fluorescence peak integration
Parallel factor (PARAFAC) analysis
Photobleaching

Access to Document

10.1016/j.scitotenv.2016.08.196

Cite this

@article{40b5a434e6ac4e77bb2d81bc5fcd2860,

title = "Fluorescence peak integration ratio IC:IT as a new potential indicator tracing the compositional changes in chromophoric dissolved organic matter",

abstract = "The present study demonstrates that the ratio of fluorescence integration of peak C to peak T (I C:I T) can be used as an indicator tracing the compositional dynamics of chromophoric dissolved organic matter (CDOM). CDOM absorption and fluorescence spectroscopy and stable isotope δ 13C were determined on a seasonal basis in seventeen Chinese inland waters as well as in a series of mixing and photodegradation experiments in the lab. A strong positive linear correlation was recorded between I C:I T and the ratio of terrestrial humic-like C1 to tryptophan-like C4 (C1:C4) derived by parallel factor analysis. The r 2 for the linear fitting between I C:I T and C1:C4 (r 2 = 0.80) was notably higher than between C1:C4 and other indices tested, including the ratio of CDOM absorption at 250 nm to 365 nm, i.e. a(250):a(365) (r 2 = 0.09), spectral slope (S 275–295) (r 2 = 0.26), spectral slope ratio (S R) (r 2 = 0.31), the humification index (HIX) (r 2 = 0.47), the recent autochthonous biological contribution index (BIX) (r 2 = 0.27), and a fluorescence index (FI 370) (r 2 = 0.07). I C:I T exhibited larger variability than the remaining six indices and a closer correlation with stable isotope δ 13C than that observed for a(250):a(365), S 275–295, S R, FI 370, and BIX during field campaigns. Confirming our field observations, significant correlations were recorded between I C:I T and the remaining six indices, and I C:I T also demonstrated notably larger variability than the six other indices during our wastewater addition experiment. Compared with HIX, eutrophic water addition and photobleaching substantially decreased I C:I T but had no pronounced effect on a(250):a(365), S 275–297, S R, FI 370, and BIX, further suggesting that I C:I T is the most efficient indicator of the CDOM compositional dynamics.",

keywords = "Chromophoric dissolved organic matter (CDOM), Fluorescence peak integration, Parallel factor (PARAFAC) analysis, Photobleaching",

author = "Yongqiang Zhou and Kun Shi and Yunlin Zhang and Erik Jeppesen and Xiaohan Liu and Qichao Zhou and Huawu Wu and Xiangming Tang and Guangwei Zhu",

year = "2016",

doi = "10.1016/j.scitotenv.2016.08.196",

language = "English",

volume = "574",

pages = "1588--1598",

journal = "Science of the Total Environment",

issn = "0048-9697",

publisher = "Elsevier B.V.",

}

Fluorescence peak integration ratio IC:IT as a new potential indicator tracing the compositional changes in chromophoric dissolved organic matter. / Zhou, Yongqiang; Shi, Kun; Zhang, Yunlin et al.
In: Science of the Total Environment, Vol. 574, 2016, p. 1588-1598.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Fluorescence peak integration ratio IC:IT as a new potential indicator tracing the compositional changes in chromophoric dissolved organic matter

AU - Zhou, Yongqiang

AU - Shi, Kun

AU - Zhang, Yunlin

AU - Jeppesen, Erik

AU - Liu, Xiaohan

AU - Zhou, Qichao

AU - Wu, Huawu

AU - Tang, Xiangming

AU - Zhu, Guangwei

PY - 2016

Y1 - 2016

N2 - The present study demonstrates that the ratio of fluorescence integration of peak C to peak T (I C:I T) can be used as an indicator tracing the compositional dynamics of chromophoric dissolved organic matter (CDOM). CDOM absorption and fluorescence spectroscopy and stable isotope δ 13C were determined on a seasonal basis in seventeen Chinese inland waters as well as in a series of mixing and photodegradation experiments in the lab. A strong positive linear correlation was recorded between I C:I T and the ratio of terrestrial humic-like C1 to tryptophan-like C4 (C1:C4) derived by parallel factor analysis. The r 2 for the linear fitting between I C:I T and C1:C4 (r 2 = 0.80) was notably higher than between C1:C4 and other indices tested, including the ratio of CDOM absorption at 250 nm to 365 nm, i.e. a(250):a(365) (r 2 = 0.09), spectral slope (S 275–295) (r 2 = 0.26), spectral slope ratio (S R) (r 2 = 0.31), the humification index (HIX) (r 2 = 0.47), the recent autochthonous biological contribution index (BIX) (r 2 = 0.27), and a fluorescence index (FI 370) (r 2 = 0.07). I C:I T exhibited larger variability than the remaining six indices and a closer correlation with stable isotope δ 13C than that observed for a(250):a(365), S 275–295, S R, FI 370, and BIX during field campaigns. Confirming our field observations, significant correlations were recorded between I C:I T and the remaining six indices, and I C:I T also demonstrated notably larger variability than the six other indices during our wastewater addition experiment. Compared with HIX, eutrophic water addition and photobleaching substantially decreased I C:I T but had no pronounced effect on a(250):a(365), S 275–297, S R, FI 370, and BIX, further suggesting that I C:I T is the most efficient indicator of the CDOM compositional dynamics.

AB - The present study demonstrates that the ratio of fluorescence integration of peak C to peak T (I C:I T) can be used as an indicator tracing the compositional dynamics of chromophoric dissolved organic matter (CDOM). CDOM absorption and fluorescence spectroscopy and stable isotope δ 13C were determined on a seasonal basis in seventeen Chinese inland waters as well as in a series of mixing and photodegradation experiments in the lab. A strong positive linear correlation was recorded between I C:I T and the ratio of terrestrial humic-like C1 to tryptophan-like C4 (C1:C4) derived by parallel factor analysis. The r 2 for the linear fitting between I C:I T and C1:C4 (r 2 = 0.80) was notably higher than between C1:C4 and other indices tested, including the ratio of CDOM absorption at 250 nm to 365 nm, i.e. a(250):a(365) (r 2 = 0.09), spectral slope (S 275–295) (r 2 = 0.26), spectral slope ratio (S R) (r 2 = 0.31), the humification index (HIX) (r 2 = 0.47), the recent autochthonous biological contribution index (BIX) (r 2 = 0.27), and a fluorescence index (FI 370) (r 2 = 0.07). I C:I T exhibited larger variability than the remaining six indices and a closer correlation with stable isotope δ 13C than that observed for a(250):a(365), S 275–295, S R, FI 370, and BIX during field campaigns. Confirming our field observations, significant correlations were recorded between I C:I T and the remaining six indices, and I C:I T also demonstrated notably larger variability than the six other indices during our wastewater addition experiment. Compared with HIX, eutrophic water addition and photobleaching substantially decreased I C:I T but had no pronounced effect on a(250):a(365), S 275–297, S R, FI 370, and BIX, further suggesting that I C:I T is the most efficient indicator of the CDOM compositional dynamics.

KW - Chromophoric dissolved organic matter (CDOM)

KW - Fluorescence peak integration

KW - Parallel factor (PARAFAC) analysis

KW - Photobleaching

UR - http://www.scopus.com/inward/record.url?scp=84994351188&partnerID=8YFLogxK

U2 - 10.1016/j.scitotenv.2016.08.196

DO - 10.1016/j.scitotenv.2016.08.196

M3 - Journal article

C2 - 27613668

SN - 0048-9697

VL - 574

SP - 1588

EP - 1598

JO - Science of the Total Environment

JF - Science of the Total Environment

ER -

Fluorescence peak integration ratio IC:IT as a new potential indicator tracing the compositional changes in chromophoric dissolved organic matter

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this