Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

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  • Anders Okholm, Department of Molecular Biology and Genetics (MBG), Aarhus University, Aarhus, Denmark Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.
  • ,
  • Jørgen Kjems
  • Kira Astakhova, Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Denmark

Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets. This journal is

Original languageEnglish
JournalRSC Advances
Volume4
Issue86
Pages (from-to)45653-45656
Number of pages4
ISSN2046-2069
DOIs
Publication statusPublished - 2014

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