Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

Anders Okholm; Jørgen Kjems; Kira Astakhova

doi:10.1039/c4ra07165d

Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

Anders Okholm, Jørgen Kjems, Kira Astakhova^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

10 Citations (Scopus)

Abstract

Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets. This journal is

Original language	English
Journal	RSC Advances
Volume	4
Issue	86
Pages (from-to)	45653-45656
Number of pages	4
ISSN	2046-2069
DOIs	https://doi.org/10.1039/c4ra07165d
Publication status	Published - 2014

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10.1039/c4ra07165d

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AU - Kjems, Jørgen

AU - Astakhova, Kira

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N2 - Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets. This journal is

AB - Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets. This journal is

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DO - 10.1039/c4ra07165d

M3 - Journal article

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SP - 45653

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JO - RSC Advances

JF - RSC Advances

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Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

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