Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors

Tine Borum Billeskov; Jonas Brorson Jensen; Niels Jessen; Jean Farup

doi:10.1016/j.xpro.2022.102008

Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors

Tine Borum Billeskov, Jonas Brorson Jensen, Niels Jessen^*, Jean Farup

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

The ability of stem cells to activate and differentiate is critical for maintaining the regenerative capacity of skeletal muscle. Here, we detail steps for specific quantification and isolation of primary human fibro-adipogenic progenitors and skeletal muscle stem cells using fluorescence-activated cell sorting. We describe important phenotypic traits such as time to enter the cell cycle and assessment of cell differentiation for the isolated cell populations. The technique has been applied on tissue obtained from surgery and needle biopsies. For complete details on the use and execution of this protocol, please refer to Farup et al. (2021).1.

Original language	English
Article number	102008
Journal	STAR Protocols
Volume	4
Issue	1
Number of pages	26
DOIs	https://doi.org/10.1016/j.xpro.2022.102008
Publication status	Published - 17 Mar 2023

Keywords

Cell Biology
Cell Differentiation
Microscopy
Stem Cells
Tissue Engineering

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10.1016/j.xpro.2022.102008Licence: CC BY-NC-ND

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title = "Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors",

abstract = "The ability of stem cells to activate and differentiate is critical for maintaining the regenerative capacity of skeletal muscle. Here, we detail steps for specific quantification and isolation of primary human fibro-adipogenic progenitors and skeletal muscle stem cells using fluorescence-activated cell sorting. We describe important phenotypic traits such as time to enter the cell cycle and assessment of cell differentiation for the isolated cell populations. The technique has been applied on tissue obtained from surgery and needle biopsies. For complete details on the use and execution of this protocol, please refer to Farup et al. (2021).1.",

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Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors. / Billeskov, Tine Borum; Jensen, Jonas Brorson; Jessen, Niels et al.
In: STAR Protocols, Vol. 4, No. 1, 102008, 17.03.2023.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors

AU - Billeskov, Tine Borum

AU - Jensen, Jonas Brorson

AU - Jessen, Niels

AU - Farup, Jean

PY - 2023/3/17

Y1 - 2023/3/17

N2 - The ability of stem cells to activate and differentiate is critical for maintaining the regenerative capacity of skeletal muscle. Here, we detail steps for specific quantification and isolation of primary human fibro-adipogenic progenitors and skeletal muscle stem cells using fluorescence-activated cell sorting. We describe important phenotypic traits such as time to enter the cell cycle and assessment of cell differentiation for the isolated cell populations. The technique has been applied on tissue obtained from surgery and needle biopsies. For complete details on the use and execution of this protocol, please refer to Farup et al. (2021).1.

AB - The ability of stem cells to activate and differentiate is critical for maintaining the regenerative capacity of skeletal muscle. Here, we detail steps for specific quantification and isolation of primary human fibro-adipogenic progenitors and skeletal muscle stem cells using fluorescence-activated cell sorting. We describe important phenotypic traits such as time to enter the cell cycle and assessment of cell differentiation for the isolated cell populations. The technique has been applied on tissue obtained from surgery and needle biopsies. For complete details on the use and execution of this protocol, please refer to Farup et al. (2021).1.

KW - Cell Biology

KW - Cell Differentiation

KW - Microscopy

KW - Stem Cells

KW - Tissue Engineering

U2 - 10.1016/j.xpro.2022.102008

DO - 10.1016/j.xpro.2022.102008

M3 - Journal article

C2 - 36640368

SN - 2666-1667

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JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 102008

ER -

Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors

Abstract

Keywords

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