Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species. / Vanthournout, Bram; Deswarte, K; Hammad, H; Bilde, Trine; Lambrecht, B; Hendrickx, F.

In: Biology Letters, Vol. 10, No. 5, 20140159, 2014.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Vanthournout, B, Deswarte, K, Hammad, H, Bilde, T, Lambrecht, B & Hendrickx, F 2014, 'Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species', Biology Letters, vol. 10, no. 5, 20140159. https://doi.org/10.1098/rsbl.2014.0159

APA

Vanthournout, B., Deswarte, K., Hammad, H., Bilde, T., Lambrecht, B., & Hendrickx, F. (2014). Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species. Biology Letters, 10(5), [20140159]. https://doi.org/10.1098/rsbl.2014.0159

CBE

Vanthournout B, Deswarte K, Hammad H, Bilde T, Lambrecht B, Hendrickx F. 2014. Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species. Biology Letters. 10(5):Article 20140159. https://doi.org/10.1098/rsbl.2014.0159

MLA

Vancouver

Vanthournout B, Deswarte K, Hammad H, Bilde T, Lambrecht B, Hendrickx F. Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species. Biology Letters. 2014;10(5). 20140159. https://doi.org/10.1098/rsbl.2014.0159

Author

Vanthournout, Bram ; Deswarte, K ; Hammad, H ; Bilde, Trine ; Lambrecht, B ; Hendrickx, F. / Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species. In: Biology Letters. 2014 ; Vol. 10, No. 5.

Bibtex

@article{2b38fe35c7f440318a0b3ea5c36e980c,
title = "Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species",
abstract = "Producing equal amounts of male and female offspring has long been considered an evolutionarily stable strategy. Nevertheless, exceptions to this general rule (i.e. male and female biases) are documented in many taxa, making sex allocation an important domain in current evolutionary biology research. Pinpointing the underlying mechanism of sex ratio bias is challenging owing to the multitude of potential sex ratio-biasing factors. In the dwarf spider, Oedothorax gibbosus, infection with the bacterial endosymbiont Wolbachia results in a female bias. However, pedigree analysis reveals that other factors influence sex ratio variation. In this paper, we investigate whether this additional variation can be explained by the unequal production of male- and female-determining sperm cells during sperm production. Using flow cytometry, we show that males produce equal amounts of male- and female-determining sperm cells; thus bias in sperm production does not contribute to the sex ratio bias observed in this species. This demonstrates that other factors such as parental genes suppressing endosymbiont effects and cryptic female choice might play a role in sex allocation in this species.",
author = "Bram Vanthournout and K Deswarte and H Hammad and Trine Bilde and B Lambrecht and F. Hendrickx",
year = "2014",
doi = "10.1098/rsbl.2014.0159",
language = "English",
volume = "10",
journal = "Biology Letters",
issn = "1744-9561",
publisher = "ROYAL SOC",
number = "5",

}

RIS

TY - JOUR

T1 - Flow cytometric sexing of spider sperm reveals an equal sperm production ratio in a female-biased species

AU - Vanthournout, Bram

AU - Deswarte, K

AU - Hammad, H

AU - Bilde, Trine

AU - Lambrecht, B

AU - Hendrickx, F.

PY - 2014

Y1 - 2014

N2 - Producing equal amounts of male and female offspring has long been considered an evolutionarily stable strategy. Nevertheless, exceptions to this general rule (i.e. male and female biases) are documented in many taxa, making sex allocation an important domain in current evolutionary biology research. Pinpointing the underlying mechanism of sex ratio bias is challenging owing to the multitude of potential sex ratio-biasing factors. In the dwarf spider, Oedothorax gibbosus, infection with the bacterial endosymbiont Wolbachia results in a female bias. However, pedigree analysis reveals that other factors influence sex ratio variation. In this paper, we investigate whether this additional variation can be explained by the unequal production of male- and female-determining sperm cells during sperm production. Using flow cytometry, we show that males produce equal amounts of male- and female-determining sperm cells; thus bias in sperm production does not contribute to the sex ratio bias observed in this species. This demonstrates that other factors such as parental genes suppressing endosymbiont effects and cryptic female choice might play a role in sex allocation in this species.

AB - Producing equal amounts of male and female offspring has long been considered an evolutionarily stable strategy. Nevertheless, exceptions to this general rule (i.e. male and female biases) are documented in many taxa, making sex allocation an important domain in current evolutionary biology research. Pinpointing the underlying mechanism of sex ratio bias is challenging owing to the multitude of potential sex ratio-biasing factors. In the dwarf spider, Oedothorax gibbosus, infection with the bacterial endosymbiont Wolbachia results in a female bias. However, pedigree analysis reveals that other factors influence sex ratio variation. In this paper, we investigate whether this additional variation can be explained by the unequal production of male- and female-determining sperm cells during sperm production. Using flow cytometry, we show that males produce equal amounts of male- and female-determining sperm cells; thus bias in sperm production does not contribute to the sex ratio bias observed in this species. This demonstrates that other factors such as parental genes suppressing endosymbiont effects and cryptic female choice might play a role in sex allocation in this species.

U2 - 10.1098/rsbl.2014.0159

DO - 10.1098/rsbl.2014.0159

M3 - Journal article

C2 - 24850893

VL - 10

JO - Biology Letters

JF - Biology Letters

SN - 1744-9561

IS - 5

M1 - 20140159

ER -