Fine tuning chloroplast movements through physical interactions between phototropins

Olga Sztatelman; Justyna Łabuz; Paweł Hermanowicz; Agnieszka Katarzyna Banaś; Aneta Bazant; Piotr Zgłobicki; Chhavi Aggarwal; Marcin Nadzieja; Weronika Krzeszowiec; Wojciech Strzałka; Halina Gabryś

doi:10.1093/jxb/erw265

Fine tuning chloroplast movements through physical interactions between phototropins

Olga Sztatelman^*
, Justyna Łabuz
, Paweł Hermanowicz
, Agnieszka Katarzyna Banaś
, Aneta Bazant
, Piotr Zgłobicki
, Chhavi Aggarwal
, Marcin Nadzieja
, Weronika Krzeszowiec
, Wojciech Strzałka
, Halina Gabryś

^*Corresponding author for this work

Department of Molecular Biology and Genetics - Plant Molecular Biology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

23 Citations (Scopus)

Abstract

Phototropins are plant photoreceptors which regulate numerous responses to blue light, including chloroplast relocation. Weak blue light induces chloroplast accumulation, whereas strong light leads to an avoidance response. Two Arabidopsis phototropins are characterized by different light sensitivities. Under continuous light, both can elicit chloroplast accumulation, but the avoidance response is controlled solely by phot2. As well as continuous light, brief light pulses also induce chloroplast displacements. Pulses of 0.1s and 0.2s of fluence rate saturating the avoidance response lead to transient chloroplast accumulation. Longer pulses (up to 20s) trigger a biphasic response, namely transient avoidance followed by transient accumulation. This work presents a detailed study of transient chloroplast responses in Arabidopsis. Phototropin mutants display altered chloroplast movements as compared with the wild type: phot1 is characterized by weaker responses, while phot2 exhibits enhanced chloroplast accumulation, especially after 0.1s and 0.2s pulses. To determine the cause of these differences, the abundance and phosphorylation levels of both phototropins, as well as the interactions between phototropin molecules are examined. The formation of phototropin homo- and heterocomplexes is the most plausible explanation of the observed phenomena. The physiological consequences of this interplay are discussed, suggesting the universal character of this mechanism that fine-tunes plant reactions to blue light. Additionally, responses in mutants of different protein phosphatase 2A subunits are examined to assess the role of protein phosphorylation in signaling of chloroplast movements.

Original language	English
Journal	Journal of Experimental Botany
Volume	67
Issue	17
Pages (from-to)	4963-4978
Number of pages	16
ISSN	0022-0957
DOIs	https://doi.org/10.1093/jxb/erw265
Publication status	Published - 1 Sept 2016

Keywords

Arabidopsis thaliana
blue light
chloroplast movements
light pulses
phototropin1
phototropin2
protein phosphatase 2A

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title = "Fine tuning chloroplast movements through physical interactions between phototropins",

abstract = "Phototropins are plant photoreceptors which regulate numerous responses to blue light, including chloroplast relocation. Weak blue light induces chloroplast accumulation, whereas strong light leads to an avoidance response. Two Arabidopsis phototropins are characterized by different light sensitivities. Under continuous light, both can elicit chloroplast accumulation, but the avoidance response is controlled solely by phot2. As well as continuous light, brief light pulses also induce chloroplast displacements. Pulses of 0.1s and 0.2s of fluence rate saturating the avoidance response lead to transient chloroplast accumulation. Longer pulses (up to 20s) trigger a biphasic response, namely transient avoidance followed by transient accumulation. This work presents a detailed study of transient chloroplast responses in Arabidopsis. Phototropin mutants display altered chloroplast movements as compared with the wild type: phot1 is characterized by weaker responses, while phot2 exhibits enhanced chloroplast accumulation, especially after 0.1s and 0.2s pulses. To determine the cause of these differences, the abundance and phosphorylation levels of both phototropins, as well as the interactions between phototropin molecules are examined. The formation of phototropin homo- and heterocomplexes is the most plausible explanation of the observed phenomena. The physiological consequences of this interplay are discussed, suggesting the universal character of this mechanism that fine-tunes plant reactions to blue light. Additionally, responses in mutants of different protein phosphatase 2A subunits are examined to assess the role of protein phosphorylation in signaling of chloroplast movements.",

keywords = "Arabidopsis thaliana, blue light, chloroplast movements, light pulses, phototropin1, phototropin2, protein phosphatase 2A",

author = "Olga Sztatelman and Justyna {\L}abuz and Pawe{\l} Hermanowicz and Bana{\'s}, \{Agnieszka Katarzyna\} and Aneta Bazant and Piotr Zg{\l}obicki and Chhavi Aggarwal and Marcin Nadzieja and Weronika Krzeszowiec and Wojciech Strza{\l}ka and Halina Gabry{\'s}",

year = "2016",

month = sep,

day = "1",

doi = "10.1093/jxb/erw265",

language = "English",

volume = "67",

pages = "4963--4978",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

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Fine tuning chloroplast movements through physical interactions between phototropins. / Sztatelman, Olga; Łabuz, Justyna; Hermanowicz, Paweł et al.
In: Journal of Experimental Botany, Vol. 67, No. 17, 01.09.2016, p. 4963-4978.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Fine tuning chloroplast movements through physical interactions between phototropins

AU - Sztatelman, Olga

AU - Łabuz, Justyna

AU - Hermanowicz, Paweł

AU - Banaś, Agnieszka Katarzyna

AU - Bazant, Aneta

AU - Zgłobicki, Piotr

AU - Aggarwal, Chhavi

AU - Nadzieja, Marcin

AU - Krzeszowiec, Weronika

AU - Strzałka, Wojciech

AU - Gabryś, Halina

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Phototropins are plant photoreceptors which regulate numerous responses to blue light, including chloroplast relocation. Weak blue light induces chloroplast accumulation, whereas strong light leads to an avoidance response. Two Arabidopsis phototropins are characterized by different light sensitivities. Under continuous light, both can elicit chloroplast accumulation, but the avoidance response is controlled solely by phot2. As well as continuous light, brief light pulses also induce chloroplast displacements. Pulses of 0.1s and 0.2s of fluence rate saturating the avoidance response lead to transient chloroplast accumulation. Longer pulses (up to 20s) trigger a biphasic response, namely transient avoidance followed by transient accumulation. This work presents a detailed study of transient chloroplast responses in Arabidopsis. Phototropin mutants display altered chloroplast movements as compared with the wild type: phot1 is characterized by weaker responses, while phot2 exhibits enhanced chloroplast accumulation, especially after 0.1s and 0.2s pulses. To determine the cause of these differences, the abundance and phosphorylation levels of both phototropins, as well as the interactions between phototropin molecules are examined. The formation of phototropin homo- and heterocomplexes is the most plausible explanation of the observed phenomena. The physiological consequences of this interplay are discussed, suggesting the universal character of this mechanism that fine-tunes plant reactions to blue light. Additionally, responses in mutants of different protein phosphatase 2A subunits are examined to assess the role of protein phosphorylation in signaling of chloroplast movements.

AB - Phototropins are plant photoreceptors which regulate numerous responses to blue light, including chloroplast relocation. Weak blue light induces chloroplast accumulation, whereas strong light leads to an avoidance response. Two Arabidopsis phototropins are characterized by different light sensitivities. Under continuous light, both can elicit chloroplast accumulation, but the avoidance response is controlled solely by phot2. As well as continuous light, brief light pulses also induce chloroplast displacements. Pulses of 0.1s and 0.2s of fluence rate saturating the avoidance response lead to transient chloroplast accumulation. Longer pulses (up to 20s) trigger a biphasic response, namely transient avoidance followed by transient accumulation. This work presents a detailed study of transient chloroplast responses in Arabidopsis. Phototropin mutants display altered chloroplast movements as compared with the wild type: phot1 is characterized by weaker responses, while phot2 exhibits enhanced chloroplast accumulation, especially after 0.1s and 0.2s pulses. To determine the cause of these differences, the abundance and phosphorylation levels of both phototropins, as well as the interactions between phototropin molecules are examined. The formation of phototropin homo- and heterocomplexes is the most plausible explanation of the observed phenomena. The physiological consequences of this interplay are discussed, suggesting the universal character of this mechanism that fine-tunes plant reactions to blue light. Additionally, responses in mutants of different protein phosphatase 2A subunits are examined to assess the role of protein phosphorylation in signaling of chloroplast movements.

KW - Arabidopsis thaliana

KW - blue light

KW - chloroplast movements

KW - light pulses

KW - phototropin1

KW - phototropin2

KW - protein phosphatase 2A

UR - https://www.scopus.com/pages/publications/84988382919

U2 - 10.1093/jxb/erw265

DO - 10.1093/jxb/erw265

M3 - Journal article

C2 - 27406783

AN - SCOPUS:84988382919

SN - 0022-0957

VL - 67

SP - 4963

EP - 4978

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 17

ER -

Fine tuning chloroplast movements through physical interactions between phototropins

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