Fibronectin adsorption, cell adhesion, and proliferation on nanostructured tantalum surfaces

A Dolatshahi-Pirouz; Thomas Hartvig Lindkjær Jensen; David Christian Kraft; Morten Foss; Peter Kingshott; John Lundsgaard Hansen; Arne Nylandsted Larsen; Jacques Chevallier; Flemming Besenbacher

doi:10.1021/nn9017872

Fibronectin adsorption, cell adhesion, and proliferation on nanostructured tantalum surfaces

A Dolatshahi-Pirouz, Thomas Hartvig Lindkjær Jensen, David Christian Kraft, Morten Foss, Peter Kingshott, John Lundsgaard Hansen, Arne Nylandsted Larsen, Jacques Chevallier, Flemming Besenbacher

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

172 Citations (Scopus)

Abstract

The interaction between dental pulp derived mesenchymal stem cells (DP-MSCs) and three different tantalum nanotopographies with and without a fibronectin coating is examined: sputter-coated tantalum surfaces with low surface roughness <0.2 nm, hut-nanostructured surfaces with a height of 2.9 +/- 0.6 nm and a width of 35 +/- 8 nm, and dome structures with a height of 13 +/- 2 nm and a width of 52 +/- 14 nm. Using ellipsometry, the adsorption and the availability of fibronectin cell-binding domains on the tantalum surfaces were examined, as well as cellular attachment, proliferation, and vinculin focal adhesion spot assembly on the respective surfaces. The results showed the highest fibronectin mass uptake on the hut structures, with a slightly higher availability of cell-binding domains and the most pronounced formation of vinculin focal adhesion spots as compared to the other surfaces. The proliferation of DP-MSCs was found to be significantly higher on dome and hut surfaces coated with fibronectin compared to the uncoated flat tantalum surfaces. Consequently, the results presented in this study indicate that fibronectin-coated nanotopographies with a vertical dimension of less than 5 nm influence cell adhesion. This rather interesting behavior is argued to originate from the more available fibronectin cell-binding domains observed on the hut structures.

Original language	English
Journal	A C S Nano
Volume	4
Issue	5
Pages (from-to)	2874-2882
Number of pages	9
ISSN	1936-0851
DOIs	https://doi.org/10.1021/nn9017872
Publication status	Published - 2010

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10.1021/nn9017872

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@article{c2d7206079ee11df8c1a000ea68e967b,

title = "Fibronectin adsorption, cell adhesion, and proliferation on nanostructured tantalum surfaces",

abstract = "The interaction between dental pulp derived mesenchymal stem cells (DP-MSCs) and three different tantalum nanotopographies with and without a fibronectin coating is examined: sputter-coated tantalum surfaces with low surface roughness <0.2 nm, hut-nanostructured surfaces with a height of 2.9 +/- 0.6 nm and a width of 35 +/- 8 nm, and dome structures with a height of 13 +/- 2 nm and a width of 52 +/- 14 nm. Using ellipsometry, the adsorption and the availability of fibronectin cell-binding domains on the tantalum surfaces were examined, as well as cellular attachment, proliferation, and vinculin focal adhesion spot assembly on the respective surfaces. The results showed the highest fibronectin mass uptake on the hut structures, with a slightly higher availability of cell-binding domains and the most pronounced formation of vinculin focal adhesion spots as compared to the other surfaces. The proliferation of DP-MSCs was found to be significantly higher on dome and hut surfaces coated with fibronectin compared to the uncoated flat tantalum surfaces. Consequently, the results presented in this study indicate that fibronectin-coated nanotopographies with a vertical dimension of less than 5 nm influence cell adhesion. This rather interesting behavior is argued to originate from the more available fibronectin cell-binding domains observed on the hut structures.",

author = "A Dolatshahi-Pirouz and Jensen, {Thomas Hartvig Lindkj{\ae}r} and Kraft, {David Christian} and Morten Foss and Peter Kingshott and Hansen, {John Lundsgaard} and Larsen, {Arne Nylandsted} and Jacques Chevallier and Flemming Besenbacher",

year = "2010",

doi = "10.1021/nn9017872",

language = "English",

volume = "4",

pages = "2874--2882",

journal = "A C S Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "5",

}

Fibronectin adsorption, cell adhesion, and proliferation on nanostructured tantalum surfaces. / Dolatshahi-Pirouz, A; Jensen, Thomas Hartvig Lindkjær; Kraft, David Christian et al.
In: A C S Nano, Vol. 4, No. 5, 2010, p. 2874-2882.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Fibronectin adsorption, cell adhesion, and proliferation on nanostructured tantalum surfaces

AU - Dolatshahi-Pirouz, A

AU - Jensen, Thomas Hartvig Lindkjær

AU - Kraft, David Christian

AU - Foss, Morten

AU - Kingshott, Peter

AU - Hansen, John Lundsgaard

AU - Larsen, Arne Nylandsted

AU - Chevallier, Jacques

AU - Besenbacher, Flemming

PY - 2010

Y1 - 2010

N2 - The interaction between dental pulp derived mesenchymal stem cells (DP-MSCs) and three different tantalum nanotopographies with and without a fibronectin coating is examined: sputter-coated tantalum surfaces with low surface roughness <0.2 nm, hut-nanostructured surfaces with a height of 2.9 +/- 0.6 nm and a width of 35 +/- 8 nm, and dome structures with a height of 13 +/- 2 nm and a width of 52 +/- 14 nm. Using ellipsometry, the adsorption and the availability of fibronectin cell-binding domains on the tantalum surfaces were examined, as well as cellular attachment, proliferation, and vinculin focal adhesion spot assembly on the respective surfaces. The results showed the highest fibronectin mass uptake on the hut structures, with a slightly higher availability of cell-binding domains and the most pronounced formation of vinculin focal adhesion spots as compared to the other surfaces. The proliferation of DP-MSCs was found to be significantly higher on dome and hut surfaces coated with fibronectin compared to the uncoated flat tantalum surfaces. Consequently, the results presented in this study indicate that fibronectin-coated nanotopographies with a vertical dimension of less than 5 nm influence cell adhesion. This rather interesting behavior is argued to originate from the more available fibronectin cell-binding domains observed on the hut structures.

AB - The interaction between dental pulp derived mesenchymal stem cells (DP-MSCs) and three different tantalum nanotopographies with and without a fibronectin coating is examined: sputter-coated tantalum surfaces with low surface roughness <0.2 nm, hut-nanostructured surfaces with a height of 2.9 +/- 0.6 nm and a width of 35 +/- 8 nm, and dome structures with a height of 13 +/- 2 nm and a width of 52 +/- 14 nm. Using ellipsometry, the adsorption and the availability of fibronectin cell-binding domains on the tantalum surfaces were examined, as well as cellular attachment, proliferation, and vinculin focal adhesion spot assembly on the respective surfaces. The results showed the highest fibronectin mass uptake on the hut structures, with a slightly higher availability of cell-binding domains and the most pronounced formation of vinculin focal adhesion spots as compared to the other surfaces. The proliferation of DP-MSCs was found to be significantly higher on dome and hut surfaces coated with fibronectin compared to the uncoated flat tantalum surfaces. Consequently, the results presented in this study indicate that fibronectin-coated nanotopographies with a vertical dimension of less than 5 nm influence cell adhesion. This rather interesting behavior is argued to originate from the more available fibronectin cell-binding domains observed on the hut structures.

U2 - 10.1021/nn9017872

DO - 10.1021/nn9017872

M3 - Journal article

C2 - 20443575

SN - 1936-0851

VL - 4

SP - 2874

EP - 2882

JO - A C S Nano

JF - A C S Nano

IS - 5

ER -

Fibronectin adsorption, cell adhesion, and proliferation on nanostructured tantalum surfaces

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