FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species

Yi Song; Andrew J. Wilson; Xue Cheng Zhang; David Thoms; Reza Sohrabi; Siyu Song; Quentin Geissmann; Yang Liu; Lauren Walgren; Sheng Yang He; Cara H. Haney

doi:10.1038/s41477-021-00914-0

FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species

Yi Song, Andrew J. Wilson, Xue Cheng Zhang, David Thoms, Reza Sohrabi, Siyu Song, Quentin Geissmann, Yang Liu, Lauren Walgren, Sheng Yang He, Cara H. Haney^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

147 Citations (Scopus)

Abstract

Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.

Original language	English
Journal	Nature Plants
Volume	7
Issue	5
Pages (from-to)	644-654
Number of pages	11
ISSN	2055-0278
DOIs	https://doi.org/10.1038/s41477-021-00914-0
Publication status	Published - May 2021
Externally published	Yes

Keywords

Arabidopsis Proteins/metabolism
Arabidopsis/metabolism
Phosphotransferases/metabolism
Pseudomonadaceae/metabolism
Pseudomonas fluorescens/metabolism
Reactive Oxygen Species/metabolism
Rhizosphere
Soil Microbiology

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10.1038/s41477-021-00914-0

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title = "FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species",

abstract = "Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.",

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author = "Yi Song and Wilson, {Andrew J.} and Zhang, {Xue Cheng} and David Thoms and Reza Sohrabi and Siyu Song and Quentin Geissmann and Yang Liu and Lauren Walgren and He, {Sheng Yang} and Haney, {Cara H.}",

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year = "2021",

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doi = "10.1038/s41477-021-00914-0",

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AU - Wilson, Andrew J.

AU - Zhang, Xue Cheng

AU - Thoms, David

AU - Sohrabi, Reza

AU - Song, Siyu

AU - Geissmann, Quentin

AU - Liu, Yang

AU - Walgren, Lauren

AU - He, Sheng Yang

AU - Haney, Cara H.

PY - 2021/5

Y1 - 2021/5

N2 - Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.

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KW - Phosphotransferases/metabolism

KW - Pseudomonadaceae/metabolism

KW - Pseudomonas fluorescens/metabolism

KW - Reactive Oxygen Species/metabolism

KW - Rhizosphere

KW - Soil Microbiology

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ER -

FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species

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Keywords

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