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Fast and Quantitative NMR Metabolite Analysis Afforded by a Paramagnetic Co-Solute

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  • Frans A.A. Mulder
  • Leonardo Tenori, University of Florence, Department of Experimental and Clinical Medicine
  • ,
  • Claudio Luchinat, University of Florence

NMR spectroscopy is an indispensable technique for the determination of the chemical identity and structure of small molecules. The method is especially recognized for its robustness and intrinsically quantitative nature, and has manifested itself as a key analytical platform for diverse fields of application, ranging from chemical synthesis to metabolomics. Unfortunately, the slow recovery of nuclear spin polarization by spin-lattice (T1) relaxation causes most experimental time to be lost on idle waiting. Furthermore, truly quantitative NMR (qNMR) spectroscopy requires waiting times of 5-times the longest T1 in the sample, making qNMR spectroscopy slow and inefficient. We demonstrate here that co-solute paramagnetic relaxation can mitigate these two problems simultaneously. The addition of a small amount of paramagnetic gadolinium chelate, available in the form of commercial contrast-agent solutions, enables cheap, quantitative, and efficient high-throughput mixture analysis.

Original languageEnglish
JournalAngewandte Chemie - International Edition
Pages (from-to)15283-15286
Number of pages4
Publication statusPublished - 2019

    Research areas

  • analytical chemistry, co-solute paramagnetic relaxation enhancement, gadolinium chelate, metabolomics, NMR spectroscopy

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