Fabrication and Characterization of Reconstituted Silk Microgels for the Storage and Release of Small Molecules

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  • Xizhou Liu, Cambridge University
  • ,
  • Zenon Toprakcioglu, Cambridge University
  • ,
  • Alexander J. Dear, Cambridge University
  • ,
  • Aviad Levin, Cambridge University
  • ,
  • Francesco Simone Ruggeri, Cambridge University
  • ,
  • Christopher G. Taylor, Cambridge University
  • ,
  • Mengsha Hu, Cambridge University
  • ,
  • Janet R. Kumita, Cambridge University
  • ,
  • Maria Andreasen
  • Christopher M. Dobson, Cambridge University
  • ,
  • Ulyana Shimanovich, Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel.
  • ,
  • Tuomas P.J. Knowles, Cambridge University

Silk fibroin is a natural protein obtained from the Bombyx mori silkworm. In addition to being the key structural component in silkworm cocoons, it also has the propensity to self-assemble in vitro into hierarchical structures with desirable properties such as high levels of mechanical strength and robustness. Furthermore, it is an appealing biopolymer due to its biocompatability, low immunogenicity, and lack of toxicity, making it a prime candidate for biomedical material applications. Here, it is demonstrated that nanofibrils formed by reconstituted silk fibroin can be engineered into supramolecular microgels using a soft lithography-based microfluidic approach. Building on these results, a potential application for these protein microgels to encapsulate and release small molecules in a controlled manner is illustrated. Taken together, these results suggest that the tailored self-assembly of biocompatible and biodegradable silk nanofibrils can be used to generate functional micromaterials for a range of potential applications in the biomedical and pharmaceutical fields.

Original languageEnglish
Article number1800898
JournalMacromolecular Rapid Communications
Publication statusPublished - Apr 2019

    Research areas

  • biopolymer, encapsulation, microfluidics, protein self-assembly, release kinetics

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