Extracellular Nanovesicle Enhanced Gene Transfection Using Polyethyleneimine in HEK293T Cells and Zebrafish Embryos

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  • Zhenzhen Zhang, China Pharmaceutical University, Jiangsu Academy of Agricultural Sciences
  • ,
  • Kai Wen, China Pharmaceutical University
  • ,
  • Chao Zhang, China Pharmaceutical University
  • ,
  • Fabrice Laroche
  • ,
  • Zhenglong Wang, China Pharmaceutical University
  • ,
  • Qiang Zhou, Nankai University
  • ,
  • Zunfeng Liu, Nankai University
  • ,
  • Jan Pieter Abrahams, University of Basel
  • ,
  • Xiang Zhou, China Pharmaceutical University, Nankai University

It is a hot topic to improve efficiency and decrease toxicity of gene transfection reagents. The extracellular nanovesicles (EVs) that are released by cells play an important role in intercellular communication and are naturally designed for genetic exchange between cells. Here, we show that the EVs have a large beneficial effect in polyethyleneimine (PEI)-mediated transfection of a GFP-encoding plasmid into HEK293T cells. An improvement of transfection efficiency of ~500% and a decrease in toxicity were observed in a specific concentration range of PEI. The EVs also greatly improved the transfection of the same plasmid into zebrafish embryos. To verify the generality of this gene transfection approach, we also tested the cell viability and gene transfection efficiency using two other plasmids (EpTEN and ELuc) and in another cell line (A549). The measured increase in transfection efficiency makes EV a promising candidate for enhancement of the quality of current PEI-based transfection technique.

Original languageEnglish
Article number448
JournalFrontiers in Bioengineering and Biotechnology
Publication statusPublished - Jun 2020

    Research areas

  • efficiency, extracellular nanovesicles, gene transfection, polyethyleneimine, zebra fish

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