Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

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DOI

  • Dan Hao
  • Bo Thomsen
  • Jiangsong Bai, China Agricultural University, Beijing Zhongnongtongchuang (ZNTC) Biotechnology Co., Ltd
  • ,
  • Shujun Peng, Northwest Agriculture and Forestry University
  • ,
  • Xianyong Lan, Northwest Agriculture and Forestry University
  • ,
  • Yongzhen Huang, Northwest Agriculture and Forestry University
  • ,
  • Xiao Wang, Technical University of Denmark
  • ,
  • Hong Chen, Northwest Agriculture and Forestry University, Technical University of Denmark

Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p <.05). We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to investigate the possible association between single-nucleotide polymorphisms (SNP) within the MXD3 gene and five different growth traits in cattle. We found two intronic SNPs (g.2694 C>T and g.3801 T>C) and one SNP in 3′untranslated region (3′UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.

Original languageEnglish
JournalVeterinary Medicine and Science
Volume6
Issue3
Pages (from-to)399-409
Number of pages11
ISSN2053-1095
DOIs
Publication statusPublished - 2020

Bibliographical note

© 2020 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd.

    Research areas

  • cattle, expression level, growth trait, MXD3 gene

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