Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

TY - JOUR

T1 - Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

AU - Hao, Dan

AU - Thomsen, Bo

AU - Bai, Jiangsong

AU - Peng, Shujun

AU - Lan, Xianyong

AU - Huang, Yongzhen

AU - Wang, Xiao

AU - Chen, Hong

N1 - © 2020 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p <.05). We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to investigate the possible association between single-nucleotide polymorphisms (SNP) within the MXD3 gene and five different growth traits in cattle. We found two intronic SNPs (g.2694 C>T and g.3801 T>C) and one SNP in 3′untranslated region (3′UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.

AB - Max dimerization protein 3 (MXD3) belongs to the MYC superfamily of basic helix-loop-helix leucine zipper transcription factors, and MXD3-MAX heterodimers can bind to promoters of target genes to modulate their expression. The aim of this study was to determine the MXD3 mRNA expression levels in various cattle tissues comprising heart, liver, spleen, lung, kidney, Longissimus dorsi muscle and subcutaneous fat in Chinese Qinchuan and Xianan cattle breeds. The RT-qPCR data showed that the MXD3 gene was variably expressed between all tissues and at levels that were significantly different between two breeds (p <.05). We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to investigate the possible association between single-nucleotide polymorphisms (SNP) within the MXD3 gene and five different growth traits in cattle. We found two intronic SNPs (g.2694 C>T and g.3801 T>C) and one SNP in 3′untranslated region (3′UTR) (g.6263 G>A) of MXD3 gene. Association analysis revealed strong associations between pairwise and triple SNP combinations and the growth traits. Based on these results, we suggest that MXD3 polymorphisms could be useful as molecular markers in the Chinese beef cattle breeding program.

KW - cattle

KW - expression level

KW - growth trait

KW - MXD3 gene

UR - http://www.scopus.com/inward/record.url?scp=85080932202&partnerID=8YFLogxK

U2 - 10.1002/vms3.251

DO - 10.1002/vms3.251

M3 - Journal article

C2 - 32141244

AN - SCOPUS:85080932202

SN - 2053-1095

VL - 6

SP - 399

EP - 409

JO - Veterinary Medicine and Science

JF - Veterinary Medicine and Science

IS - 3

ER -