Expression of NK cell and monocyte receptors in critically ill patients - potential biomarkers of sepsis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Expression of NK cell and monocyte receptors in critically ill patients - potential biomarkers of sepsis. / Kjaergaard, A G; Nielsen, Jeppe Sylvest; Tønnesen, Else; Krog, Jan.

In: Scandinavian Journal of Immunology, Vol. 81, No. 4, 04.2015, p. 249-58.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Kjaergaard, A G ; Nielsen, Jeppe Sylvest ; Tønnesen, Else ; Krog, Jan. / Expression of NK cell and monocyte receptors in critically ill patients - potential biomarkers of sepsis. In: Scandinavian Journal of Immunology. 2015 ; Vol. 81, No. 4. pp. 249-58.

Bibtex

@article{6e034536cf88462f976dae8cffeffde4,
title = "Expression of NK cell and monocyte receptors in critically ill patients - potential biomarkers of sepsis",
abstract = "UNLABELLED: Sepsis is characterized by activation of both the innate and adaptive immune systems as a response to infection. During sepsis, the expression of surface receptors expressed on immune competent cells, such as NKG2D and NKp30 on NK cells and TLR4 and CD14 on monocytes, is partly regulated by pro- and anti-inflammatory mediators. In this observational study, we aimed to explore whether the expression of these receptors could be used as diagnostic and/or prognostic biomarkers in sepsis. Patients with severe sepsis or septic shock (n = 21) were compared with critically ill non-septic patients (n = 15). Healthy volunteers (n = 15) served as controls. To elucidate variations over time, all patients were followed for 4 days. Cell surface expression of NKG2D, NKp30, TLR4 and CD14 and serum levels of IL-1β, IL-6, IFN-γ, TNF-α, IL-4 and IL-10 was estimated by flow cytometry. We found that NK cell expression of NKG2D and monocyte expression of CD14 were lower in the septic patients compared with the non-septic patients, both at ICU admission and during the observation period (P < 0.01 for all comparisons). Both at ICU admission, and during the observation period, levels of IL-6 and IL-10 were higher in the septic patients compared with the non-septic patients (P < 0.001 for all comparisons).CONCLUSION: As both NKG2D and CD14 levels appear to distinguish between septic and non-septic patients, both NKG2D and CD14 may be considered potential diagnostic biomarkers of severe sepsis and septic shock.",
keywords = "Aged, Antigens, CD14, Biomarkers, Female, Flow Cytometry, Humans, Interferon-gamma, Interleukin-10, Interleukin-1beta, Interleukin-6, Killer Cells, Natural, Leukocytes, Mononuclear, Male, Middle Aged, NK Cell Lectin-Like Receptor Subfamily K, Natural Cytotoxicity Triggering Receptor 3, Sepsis, Statistics, Nonparametric, Toll-Like Receptor 4, Tumor Necrosis Factor-alpha",
author = "Kjaergaard, {A G} and Nielsen, {Jeppe Sylvest} and Else T{\o}nnesen and Jan Krog",
note = "{\textcopyright} 2015 John Wiley & Sons Ltd.",
year = "2015",
month = apr,
doi = "10.1111/sji.12272",
language = "English",
volume = "81",
pages = "249--58",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Expression of NK cell and monocyte receptors in critically ill patients - potential biomarkers of sepsis

AU - Kjaergaard, A G

AU - Nielsen, Jeppe Sylvest

AU - Tønnesen, Else

AU - Krog, Jan

N1 - © 2015 John Wiley & Sons Ltd.

PY - 2015/4

Y1 - 2015/4

N2 - UNLABELLED: Sepsis is characterized by activation of both the innate and adaptive immune systems as a response to infection. During sepsis, the expression of surface receptors expressed on immune competent cells, such as NKG2D and NKp30 on NK cells and TLR4 and CD14 on monocytes, is partly regulated by pro- and anti-inflammatory mediators. In this observational study, we aimed to explore whether the expression of these receptors could be used as diagnostic and/or prognostic biomarkers in sepsis. Patients with severe sepsis or septic shock (n = 21) were compared with critically ill non-septic patients (n = 15). Healthy volunteers (n = 15) served as controls. To elucidate variations over time, all patients were followed for 4 days. Cell surface expression of NKG2D, NKp30, TLR4 and CD14 and serum levels of IL-1β, IL-6, IFN-γ, TNF-α, IL-4 and IL-10 was estimated by flow cytometry. We found that NK cell expression of NKG2D and monocyte expression of CD14 were lower in the septic patients compared with the non-septic patients, both at ICU admission and during the observation period (P < 0.01 for all comparisons). Both at ICU admission, and during the observation period, levels of IL-6 and IL-10 were higher in the septic patients compared with the non-septic patients (P < 0.001 for all comparisons).CONCLUSION: As both NKG2D and CD14 levels appear to distinguish between septic and non-septic patients, both NKG2D and CD14 may be considered potential diagnostic biomarkers of severe sepsis and septic shock.

AB - UNLABELLED: Sepsis is characterized by activation of both the innate and adaptive immune systems as a response to infection. During sepsis, the expression of surface receptors expressed on immune competent cells, such as NKG2D and NKp30 on NK cells and TLR4 and CD14 on monocytes, is partly regulated by pro- and anti-inflammatory mediators. In this observational study, we aimed to explore whether the expression of these receptors could be used as diagnostic and/or prognostic biomarkers in sepsis. Patients with severe sepsis or septic shock (n = 21) were compared with critically ill non-septic patients (n = 15). Healthy volunteers (n = 15) served as controls. To elucidate variations over time, all patients were followed for 4 days. Cell surface expression of NKG2D, NKp30, TLR4 and CD14 and serum levels of IL-1β, IL-6, IFN-γ, TNF-α, IL-4 and IL-10 was estimated by flow cytometry. We found that NK cell expression of NKG2D and monocyte expression of CD14 were lower in the septic patients compared with the non-septic patients, both at ICU admission and during the observation period (P < 0.01 for all comparisons). Both at ICU admission, and during the observation period, levels of IL-6 and IL-10 were higher in the septic patients compared with the non-septic patients (P < 0.001 for all comparisons).CONCLUSION: As both NKG2D and CD14 levels appear to distinguish between septic and non-septic patients, both NKG2D and CD14 may be considered potential diagnostic biomarkers of severe sepsis and septic shock.

KW - Aged

KW - Antigens, CD14

KW - Biomarkers

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Interferon-gamma

KW - Interleukin-10

KW - Interleukin-1beta

KW - Interleukin-6

KW - Killer Cells, Natural

KW - Leukocytes, Mononuclear

KW - Male

KW - Middle Aged

KW - NK Cell Lectin-Like Receptor Subfamily K

KW - Natural Cytotoxicity Triggering Receptor 3

KW - Sepsis

KW - Statistics, Nonparametric

KW - Toll-Like Receptor 4

KW - Tumor Necrosis Factor-alpha

U2 - 10.1111/sji.12272

DO - 10.1111/sji.12272

M3 - Journal article

C2 - 25619264

VL - 81

SP - 249

EP - 258

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 4

ER -