Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). / Studer, Bruno; Asp, Torben; Frei, Ursula; Hentrup, Stephan; Meally, Helena; Guillard, Aurélie; Barth, Susanne; Muylle, Hilde; Roldán-Ruiz, Isabel; Barre, Philippe; Koning-Boucoiran, Carole; Uenk-Stunnenberg, Gerda; Dolstra, Oene; Skøt, Leif; Skøt, Kirsten P; Turner, Lesley B; Humphreys, Mervyn O; Kölliker, Roland; Roulund, Niels; Nielsen, Klaus K; Lübberstedt, Thomas.

In: Molecular Breeding, Vol. 21, No. 4, 2008, p. 533-548.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Studer, B, Asp, T, Frei, U, Hentrup, S, Meally, H, Guillard, A, Barth, S, Muylle, H, Roldán-Ruiz, I, Barre, P, Koning-Boucoiran, C, Uenk-Stunnenberg, G, Dolstra, O, Skøt, L, Skøt, KP, Turner, LB, Humphreys, MO, Kölliker, R, Roulund, N, Nielsen, KK & Lübberstedt, T 2008, 'Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)', Molecular Breeding, vol. 21, no. 4, pp. 533-548. https://doi.org/10.1007/s11032-007-9148-0

APA

Studer, B., Asp, T., Frei, U., Hentrup, S., Meally, H., Guillard, A., Barth, S., Muylle, H., Roldán-Ruiz, I., Barre, P., Koning-Boucoiran, C., Uenk-Stunnenberg, G., Dolstra, O., Skøt, L., Skøt, K. P., Turner, L. B., Humphreys, M. O., Kölliker, R., Roulund, N., ... Lübberstedt, T. (2008). Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Molecular Breeding, 21(4), 533-548. https://doi.org/10.1007/s11032-007-9148-0

CBE

Studer B, Asp T, Frei U, Hentrup S, Meally H, Guillard A, Barth S, Muylle H, Roldán-Ruiz I, Barre P, Koning-Boucoiran C, Uenk-Stunnenberg G, Dolstra O, Skøt L, Skøt KP, Turner LB, Humphreys MO, Kölliker R, Roulund N, Nielsen KK, Lübberstedt T. 2008. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Molecular Breeding. 21(4):533-548. https://doi.org/10.1007/s11032-007-9148-0

MLA

Vancouver

Author

Studer, Bruno ; Asp, Torben ; Frei, Ursula ; Hentrup, Stephan ; Meally, Helena ; Guillard, Aurélie ; Barth, Susanne ; Muylle, Hilde ; Roldán-Ruiz, Isabel ; Barre, Philippe ; Koning-Boucoiran, Carole ; Uenk-Stunnenberg, Gerda ; Dolstra, Oene ; Skøt, Leif ; Skøt, Kirsten P ; Turner, Lesley B ; Humphreys, Mervyn O ; Kölliker, Roland ; Roulund, Niels ; Nielsen, Klaus K ; Lübberstedt, Thomas. / Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). In: Molecular Breeding. 2008 ; Vol. 21, No. 4. pp. 533-548.

Bibtex

@article{d25432f09de411dcbe9e000ea68e967b,
title = "Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)",
abstract = "An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.",
keywords = "Comparative mapping, Expressed sequence tag (EST), Ryegrass (Lolium spp.), Simple sequence repeat (SSR)",
author = "Bruno Studer and Torben Asp and Ursula Frei and Stephan Hentrup and Helena Meally and Aur{\'e}lie Guillard and Susanne Barth and Hilde Muylle and Isabel Rold{\'a}n-Ruiz and Philippe Barre and Carole Koning-Boucoiran and Gerda Uenk-Stunnenberg and Oene Dolstra and Leif Sk{\o}t and Sk{\o}t, {Kirsten P} and Turner, {Lesley B} and Humphreys, {Mervyn O} and Roland K{\"o}lliker and Niels Roulund and Nielsen, {Klaus K} and Thomas L{\"u}bberstedt",
year = "2008",
doi = "10.1007/s11032-007-9148-0",
language = "English",
volume = "21",
pages = "533--548",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

AU - Studer, Bruno

AU - Asp, Torben

AU - Frei, Ursula

AU - Hentrup, Stephan

AU - Meally, Helena

AU - Guillard, Aurélie

AU - Barth, Susanne

AU - Muylle, Hilde

AU - Roldán-Ruiz, Isabel

AU - Barre, Philippe

AU - Koning-Boucoiran, Carole

AU - Uenk-Stunnenberg, Gerda

AU - Dolstra, Oene

AU - Skøt, Leif

AU - Skøt, Kirsten P

AU - Turner, Lesley B

AU - Humphreys, Mervyn O

AU - Kölliker, Roland

AU - Roulund, Niels

AU - Nielsen, Klaus K

AU - Lübberstedt, Thomas

PY - 2008

Y1 - 2008

N2 - An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.

AB - An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.

KW - Comparative mapping

KW - Expressed sequence tag (EST)

KW - Ryegrass (Lolium spp.)

KW - Simple sequence repeat (SSR)

U2 - 10.1007/s11032-007-9148-0

DO - 10.1007/s11032-007-9148-0

M3 - Journal article

VL - 21

SP - 533

EP - 548

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

IS - 4

ER -