Exploring Bioactive Compounds in Anaerobically Digested Slurry: Extraction, Characterization, and Assessment of Antifungal Activity

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  • Jiaxin Lu, China Agricultural University, China
  • Atif Muhmood, China Agricultural University, China
  • Hongtao Liu, Chinese Academy of Sciences, China
  • Renjie Dong, China Agricultural University, China
  • Sen Pang, China Agricultural University, China
  • Shubiao Wu

Anaerobically digested slurry is well known for its use as a bio-fertilizer because of its high macro-nutrient content (e.g. N, P, K), which is essential for plant growth. The suppressive effect of slurry land application on pathogenic fungal growth due to the presence of bioactive and antimicrobial substances generated during anaerobic digestion has recently been reported. However, knowledge about the identification, characterization, and subsequent antimicrobial activity evaluation of various bioactive compounds in the anaerobically digested slurry is still insufficient. Ultrasound-assisted extraction using three solvents, ethyl acetate (EA), dichloromethane (DM), and n-butanol (NB), was employed for extraction of bioactive compounds. After extraction and characterization, antifungal activity against Fusarium oxysporum was assessed. EA was found to be a more efficient extractant with less evaporating time (4 min), irrespective of temperature. DM was found to be efficient in the extraction of O-heterocycles, while N-heterocycles were enriched in EA extracts. Furthermore, the bioactive compounds, 1,2-benzenedicarboxylic acid, butyl 2-methylpropyl ester (54.9%), and 9-octadecenamide, (Z)-(2.51%) were detected in the EA extract, while tetrahydro-2-furanmethanamine (1.32%), cyclic octatomic sulfur (1.17%), squalene (4.06%), and cholestan-3-ol (9.06%) were detected in the DM extract. The EA extract achieved approximately 84% inhibition of F. oxysporum, while only 63% inhibition was observed with the DM extract. Collectively, these findings indicate that in addition to its role as a fertilizer, digested slurry may also contribute to the control of phytopathogens upon land application because of the presence of various bioactive compounds. However, further work is needed to explore the diversity of bioactive compounds in digested manures and evaluate their antimicrobial potential. Graphical Abstract: [Figure not available: see fulltext.].

Original languageEnglish
JournalWaste and Biomass Valorization
Volume11
Pages (from-to)1863-1872
Number of pages10
ISSN1877-2641
DOIs
Publication statusPublished - May 2020

    Research areas

  • Bioactive compounds, Extraction, Heterocycles, Phytopathogens, Ultrasound

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