Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite-host cross talk

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Eline P Hansen, Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark
  • ,
  • Bastian Fromm, Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 17121 Stockholm, Sweden.
  • ,
  • Sidsel D Andersen
  • Antonio Marcilla, Joint Unit on Endocrinology, Nutrition and Clinical Dietetics, Instituto de Investigación Sanitaria-La Fe Valencia, València, Spain.
  • ,
  • Kasper L Andersen, Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark
  • ,
  • Anne Borup
  • Andrew R Williams, Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark
  • ,
  • Aaron R Jex, Population Health and Immunity Division, The Walter and Eliza Hall Institute, Parkville, Victoria 3010, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
  • ,
  • Robin B Gasser, Population Health and Immunity Division, The Walter and Eliza Hall Institute, Parkville, Victoria 3010, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
  • ,
  • Neil D Young, Population Health and Immunity Division, The Walter and Eliza Hall Institute, Parkville, Victoria 3010, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
  • ,
  • Ross S Hall, Population Health and Immunity Division, The Walter and Eliza Hall Institute, Parkville, Victoria 3010, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
  • ,
  • Allan Stensballe
  • Vladimir Ovchinnikov, Department of Human and Animal Genetics, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation.
  • ,
  • Yan Yan
  • Merete Fredholm, Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark
  • ,
  • Stig M Thamsborg, Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark
  • ,
  • Peter Nejsum

The prevalent porcine helminth, Ascaris suum, compromises pig health and reduces farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people representing a disease burden of 1.31 million disability-adjusted life years. The infections are often chronic in nature, and the parasites have a profound ability to modulate their hosts' immune responses. This study provides the first in-depth characterisation of extracellular vesicles (EVs) from different developmental stages and body parts of A. suum and proposes the role of these vesicles in the host-parasite interplay. The release of EVs from the third- (L3) and fourth-stage (L4) larvae and adults was demonstrated by transmission electron microscopy (TEM), and sequencing of EV-derived RNA identified a number of microRNAs (miRNAs) and transcripts of potential host immune targets, such as IL-13, IL-25 and IL-33, were identified. Furthermore, proteomics of EVs identified several proteins with immunomodulatory properties and other proteins previously shown to be associated with parasite EVs. Taken together, these results suggest that A. suum EVs and their cargo may play a role in host-parasite interactions. This knowledge may pave the way to novel strategies for helminth infection control and knowledge of their immune modulatory potential.

Original languageEnglish
Article number1578116
JournalJournal of Extracellular Vesicles
Volume8
Issue1
Pages (from-to)1578116
Number of pages13
ISSN2001-3078
DOIs
Publication statusPublished - 2019

    Research areas

  • Ascaris suum, extracellular vesicles, host–parasite interactions, immunity, miRNA, proteomics

See relations at Aarhus University Citationformats

ID: 145948030