Explant cultures of human colon

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Herman Autrup, Denmark
  • L.A. Barrett, United States
  • F.E. Jackson, United States
  • M.L. Jesusdason, United States
  • G.D. Stoner, United States
  • P. Phelps, United States
  • B.F. Trump, United States
  • C.C. Harris, United States
  • Institute of Environmental and Occupational Medicine
Human colonic epithelium has been cultured as explants in a chemically defined medium for periods of 1 to 20 days. The viability of the explants was shown by the preservation of the ultrastructural features of the colonic epithelial cells and by active incorporation of radioactive precursors into cellular DNA and protein. A progressive decrease in the number of goblet cells, decrease in the depth of the crypts, and a change from a columnar to a cuboidal epithelium were observed. After 20 days in culture the colonic mucosa consisted of a single layer of cuboidal epithelial cells and a few glands. The ability to maintain colonic mucosa in culture was subject to both intra- and interindividual variation. Cultured human colonic mucosa also activated a chemical procarcinogen, benzo[a]pyrene, into metabolites which bound to cellular DNA. A 100-fold interindividual variation in this binding was observed.
Original languageEnglish
Pages (from-to)1248-1257
Number of pages10
Publication statusPublished - Jun 1978

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