Explant culture of rat colon: A model system for studying metabolism of chemical carcinogens

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearch

  • Herman Autrup
  • G.D. Stoner, Denmark
  • F. Jackson, Denmark
  • C.C. Harris, Denmark
  • A.K.M. Shamsuddin, Denmark
  • L.A. Barrett, Denmark
  • B.F. Trump, Denmark
  • Institute of Environmental and Occupational Medicine
An explant culture system has been developed for the long-term maintenance of colonic tissue from the rat. Explants of 1 cm2 in size were placed in tissue-culture dishes to which was added 2 ml of CMRL-1066 medium supplemented with glucose, hydrocortisone, beta-retinyl acetate, and either 2.5% bovine albumin or 5% fetal bovine serum. The dishes were placed in a controlled-atmosphere chamber which was gassed with 95% O2 and 5% CO2. The chamber then was placed on a rocker platform which rocked at 10 cycles per min causing the medium to flow intermittently over the epithelial surface. The explants were incubated at 30 degrees C. The viability of the tissue was measured both by incorporation of specific precursors into cellular macromolecules and by monitoring of tissue morphology with light and electron microscopy. Cultured rat colon was able to metabolize benzo[alpha]pyrene, 7,12-dimethylbenz[alpha]anthracene, aflatoxin B1, dimethylnitrosamine, 1,2-dimethylhydrazine, and methylazoxymethanol acetate into chemical species that bind to cellular DNA and protein.
Original languageEnglish
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume14
Issue10
Pages (from-to)868-877
Number of pages10
ISSN1071-2690
Publication statusPublished - Oct 1978

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